MAX antibody (AA 30-106)

Catalog No. ABIN5692891

Product Details anti-MAX Antibody

Target: MAX (MYC Associated Factor X (MAX))

Binding Specificity: AA 30-106

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAX antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Brand: Picoband™

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for MAX detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.

Immunogen: E. coli-derived human MAX recombinant protein (Position: A30-R106).

Application Details

Application Notes:

Recommended Detection Systems: Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Application Details: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 μg/mL
Direct ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Target Details for MAX

Target: MAX (MYC Associated Factor X (MAX))

Alternative Name: MAX (MAX Products)

Synonyms: CG9648 antibody, Dmel\\CG9648 antibody, MAX antibody, bHLHd9 antibody, dMax antibody, dmax antibody, max antibody, bhlhd4 antibody, bhlhd5 antibody, bhlhd6 antibody, bhlhd7 antibody, bhlhd8 antibody, xmax2 antibody, xmax4 antibody, xmax antibody, AA960152 antibody, AI875693 antibody, bHLHd4 antibody, bHLHd5 antibody, bHLHd6 antibody, bHLHd7 antibody, bHLHd8 antibody, fa92b04 antibody, fb52a04 antibody, fb95h11 antibody, wu:fa92b04 antibody, wu:fb52a04 antibody, wu:fb95h11 antibody, zmax antibody, CG9648 gene product from transcript CG9648-RD antibody, MYC associated factor X L homeolog antibody, MYC associated factor X antibody, Max protein antibody, MYC associated factor X S homeolog antibody, myc associated factor X antibody, Max antibody, max.L antibody, MAX antibody, max antibody, max.S antibody

Background:

Synonyms: Protein max, Class D basic helix-loop-helix protein 4, bHLHd4, Myc-associated factor X, MAX, BHLHD4

Tissue Specificity: High levels found in the brain, heart and lung while lower levels are seen in the liver, kidney and skeletal muscle.

Background: MAX(Max protein), also called Myc-associated factor x, is the most conserved dimerization component of the MYC-MAX-MXD1 network of basic helix-loop-helix leucine zipper (bHLHZ) transcription factors that regulate cell proliferation, differentiation, and apoptosis. The conservation of the MAX sequence is particularly high in the bHLHZ domain, which is involved in protein-protein interactions and DNA binding. The MAX gene is located on chromosome 14q23 by fluorescence in situ chromosomal hybridization. Both quasisymmetric heterodimers resemble the symmetric MAX homodimer, albeit with marked structural differences in the coiled-coil leucine zipper regions that explain preferential homo- and heteromeric dimerization of these 3 evolutionarily related DNA-binding proteins. MAX acts as a classic tumor suppressor gene. Normal lymphocytes from patients showed absence of methylation of the MAX promoter and biallelic expression of MAX, which ruled out an imprinting-mediated effect on MAX expression. The ability of these cells to divide, differentiate, and apoptose in the absence of Max demonstrated for the first time that these processes can occur via Max- and possibly Myc-independent mechanisms.

UniProt: P61244

Pathways: Mitotic G1-G1/S Phases