Phospholipase C gamma 2 antibody (AA 1201-1258)

Catalog No. ABIN5692854

Product Details anti-Phospholipase C gamma 2 Antibody

Target: Phospholipase C gamma 2 (PLCG2)

Binding Specificity: AA 1201-1258

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phospholipase C gamma 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for PLCG 2 detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.

Immunogen: E. coli-derived human PLCG 2 recombinant protein (Position: R1201-V1258).

Application Details

Application Notes:

Recommended Detection Systems: Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Application Details: Western blot,0.1-0.5 μg/mL
Immunohistochemistry(Paraffin-embedded Section),0.5-1 μg/mL
Direct ELISA,0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Target Details for Phospholipase C gamma 2

Target: Phospholipase C gamma 2 (PLCG2)

Alternative Name: PLCG2 (PLCG2 Products)

Synonyms: PLCgamma2 antibody, Plcg-2 antibody, APLAID antibody, FCAS3 antibody, phospholipase C, gamma 2 antibody, phospholipase C gamma 2 antibody, phospholipase C, gamma 2 (phosphatidylinositol-specific) antibody, Plcg2 antibody, PLCG2 antibody

Background:

Synonyms: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2, Phosphoinositide phospholipase C-gamma-2, Phospholipase C-IV, PLC-IV, Phospholipase C-gamma-2, PLC-gamma-2, PLCG2

Background: 1-Phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-2 is an enzyme that in humans is encoded by the PLCG2 gene. The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3.

UniProt: P16885

Pathways: RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades, VEGF Signaling, BCR Signaling