This Estrogen Receptor alpha antibody is un-conjugated
Application
Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
Specificity
The antibody AER304 is directed against estrogen receptor alpha (ERα) and recognises amino acids residues 120 - 170, an epitope located in the B domain of ERα.
Immunogen
AER304 is a mouse monoclonal IgG1 antibody derived by fusion of Sp2/0-Ag14 mouse myeloma cells with splenocytes from a BALB/c mouse, immunized with estrogen receptor protein purified from calf uterus.
ESR1
Reactivity: Human, Mouse, Rat
WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
The AER304 antibody is suitable for the detection of estrogen receptor alpha by western blotting, immunoprecipitation and immunocytochemistry. In western blotting, the antibody recognises a doublet of approximately 67 kDa in non-reducing conditions. Optimal antibody dilutions for the different applications should be determined by titration.
Estrogen receptor (ER) is a hormone binding molecule, which belongs to the nuclear hormone family of intracellular receptors. Estrogen receptors exist as alpha and beta forms encoded by separate genes ESR1 and ESR2. ERα and ERβ share a high degree of sequence homology and are activated by the hormone 17β-estradiol and related compounds, including phytoestrogens and xenoestrogens. Both ERα and ERβ function as transcription factors which mediate many of the biological effects of estradiol at the level of gene regulation. Both receptors and are involved in the normal development and function of numerous tissues. Studies also suggest that ER's may be involved in a range of pathological conditions including cancers, neurodegenerative diseases and cardiovascular disease.