Heregulin beta 1 antibody
Reactivity: Human
ELISA, WB, Neut
Host: Rabbit
Polyclonal
unconjugated
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- Target See all Heregulin beta 1 products
- Heregulin beta 1
- Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Heregulin beta 1 antibody is un-conjugated
- Application
- ELISA, Western Blotting (WB), Neutralization (Neut)
- Immunogen
- Produced from sera of rabbits immunized with highly pure recombinant Human Heregulin-B1. Human Heregulin-B1 specific antibody was purified by affinity chromatography employing an immobilized Human Heregulin-B1 matrix.
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- Application Notes
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Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hHeregulin-B1 (1.0 ng/mL), a concentration of 0.1-1.1 μ,g/mL of this antibody is required.
ELISA:
In a sandwich ELISA To detect hHeregulin-B1 (using 100 uL/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction withour biotinylated Anti-Human hHeregulin-B1as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hHeregulin-B1.
Western Blot:
To detect hHeregulin-B1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hHeregulin-B1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Storage
- -20 °C
- Storage Comment
- The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- Target
- Heregulin beta 1
- Alternative Name
- Heregulin-beta1 (Heregulin beta 1 Products)
- Gene ID
- 3084
- UniProt
- Q02297
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