C19orf10 antibody (Biotin)

Catalog No. ABIN5690391

Product Details anti-C19orf10 Antibody (Biotin)

Target: C19orf10 (Chromosome 19 Open Reading Frame 10 (C19orf10))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This C19orf10 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mSF-20. Murine SF-20 specific antibody was purified by affinity chromatography and then biotinylated.

Application Details

Application Notes: ELISA:
Direct:
To detect mSF-20 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSF-20.

Sandwich
To detect mSF-20 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Murine SF-20 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSF-20.

Western Blot:
To detect mSF-20 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSF-20 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: SF-20 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for C19orf10

Target: C19orf10 (Chromosome 19 Open Reading Frame 10 (C19orf10))

Alternative Name: SF-20 (C19orf10 Products)

Background: SF20, originally identified as a product of bone marrow-derived stromal cells, was previously thought to support proliferation of lymphoid cells and was designated as interleukin. However, this activity has not been reproducible and the function of this protein is currently unknown

Gene ID: 28106

UniProt: Q9CPT4

Pathways: ER-Nucleus Signaling