TRIP12 antibody (AA 1930-1980)

Catalog No. ABIN5690304

Product Details anti-TRIP12 Antibody

Target: TRIP12 (Thyroid Hormone Receptor Interactor 12 (TRIP12))

Binding Specificity: AA 1930-1980

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIP12 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity: TRIP12 antibody is human, mouse and rat reactive. At least four isoforms are known to exist.

Purification: TRIP12 antibody is affinity chromatography purified via peptide column.

Immunogen: TRIP12 antibody was raised against a 18 amino acid peptide near the carboxy terminus of human TRIP12.

The immunogen is located within amino acids 1930 - 1980 of TRIP12.

Isotype: IgG

Application Details

Application Notes: TRIP12 antibody can be used for detection of TRIP12 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: TRIP12 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: TRIP12 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for TRIP12

Target: TRIP12 (Thyroid Hormone Receptor Interactor 12 (TRIP12))

Alternative Name: TRIP12 (TRIP12 Products)

Synonyms: ULF antibody, TRIP-12 antibody, 1110036I07Rik antibody, 6720416K24Rik antibody, AA410158 antibody, Gtl6 antibody, thyroid hormone receptor interactor 12 antibody, E3 ubiquitin-protein ligase TRIP12 antibody, TRIP12 antibody, CpipJ_CPIJ012812 antibody, PTRG_06487 antibody, LOAG_11488 antibody, LOC100631469 antibody, Trip12 antibody

Background: Thyroid hormone receptors (TRs) are transcription factors that regulate the expression of specific genes in a hormone-dependent manner (1). TRIP12 (thyroid hormone receptor interactor 12) is an ATP-dependent E3 ubiquitin ligase involved in the human ubiquitin fusion degradation (UFD) pathway and also modulates the NEDD8 pathway (2,3). TRIP12 contains one WWE domain and a single HECT (E6AP-type E3 ubiquitin-protein ligase) domain suggested to contain a noncovalent ubiquitin-binding site (4). TRIP12 acts as a key regulator of DNA damage response and the ubiquitin ligase activity of TRIP12 is essential for mouse development (5).

Molecular Weight: Predicted: 219 kDa

Observed: 220 kDa

Gene ID: 9320

NCBI Accession: NP_004229

UniProt: Q14669

Pathways: Nuclear Hormone Receptor Binding