Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Specificity
KLF17 antibody is human, mouse and rat reactive. At least two isoforms of KLF17 are known to exist, this antibody will only detect the large isoform. KLF17 antibody is predicted to not cross-react with other KLF proteins.
Purification
KLF17 antibody is affinity chromatography purified via peptide column.
Immunogen
KLF17 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human KLF17.
The immunogen is located within the last 50 amino acids of KLF17.
KLF17
Reactivity: Human
WB, IF (cc), IF (p)
Host: Rabbit
Polyclonal
AbBy Fluor® 750
Application Notes
KLF17 antibody can be used for detection of KLF17 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
KLF17 antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
KLF17 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
KLF17, also known as ZNF393, is a member of the Sp/KLF family of transcription factors (1,2). KLF17 binds to the promoter and negatively regulates the transcription of Id-1, a key metastasis regulator in breast cancer (3). Suppression of KLF17 promotes breast cancer cell invasion and epithelial-mesenchymal transition (3). Down-regulated KLF17 expression is also associated tumor invasion and poor prognosis in hepatocellular carcinoma, suggesting that the level of KLF17 expression can serve as a prognostic indicator (4).