DNAJC13 antibody (AA 1940-1990)

Catalog No. ABIN5689948

Product Details anti-DNAJC13 Antibody

Target: DNAJC13 (DnaJ heat shock protein family (Hsp40) member C13 (DNAJC13))

Binding Specificity: AA 1940-1990

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNAJC13 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: DNAJC13 antibody is human and mouse reactive. At least two siforms of DNAJC13 are known to exist, this antibody will detect both isoforms. DNAJC13 antibody is predicted to not cross-react with other DNAJC family members.

Purification: DNAJC13 antibody is affinity chromatography purified via peptide column.

Immunogen: DNAJC13 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DNAJC13.

The immunogen is located within amino acids 1940 - 1990 of DNAJC13.

Isotype: IgG

Application Details

Application Notes: DNAJC13 antibody can be used for detection of DNAJC13 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: DNAJC13 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: DNAJC13 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for DNAJC13

Target: DNAJC13 (DnaJ heat shock protein family (Hsp40) member C13 (DNAJC13))

Alternative Name: DNAJC13 (DNAJC13 Products)

Synonyms: RME8 antibody, D030002L11Rik antibody, Gm1124 antibody, RME-8 antibody, Rme8 antibody, mKIAA0678 antibody, DnaJ heat shock protein family (Hsp40) member C13 antibody, dnaJ homolog subfamily C member 13 antibody, DNAJC13 antibody, LOC100167963 antibody, LOC100640734 antibody, Dnajc13 antibody

Background: DNAJC13, also known as receptor-mediated endocytosis 8 (RME8), is the human homolog to a DnaJ domain-containing protein originally identified in a screen for endocytic defects in C. elegans (1). It is thought to be a co-chaperone of Hsc70 which regulates protein conformation at membrane sites and plays a role in intracellular trafficking, co-localizing with markers of the endosomal system. Recent experiments have indicated that the DNAJC13 protein is involved in membrane trafficking through early endosomes but not through degradative organelles (2). DNAJC13 has been also been shown to regulate the intracellular trafficking of the epidermal growth factor receptor (3).

Molecular Weight: Predicted: 249 kDa

Observed: 249 kDa

Gene ID: 23317

NCBI Accession: NP_056083

UniProt: O75165