Cofilin1/2 (CFL1/2) (pTyr88) antibody

Catalog No. ABIN5689861

Product Details

Target: Cofilin1/2 (CFL1/2)

Binding Specificity: pTyr88

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: This antibody detects endogenous level of cofilin1/cofilin2 only when phosphorylated at tyrosine 88.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Immunogen: cofilin1/cofilin2 (phospho-Tyr88) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 88 (A-T-Y (p) -E-T) derived from Human coflin1/cofilin2.

Application Details

Application Notes: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details

Target: Cofilin1/2 (CFL1/2)

Alternative Name: cofilin1 / cofilin2 (CFL1/2 Products)

Background: Controls reversibly actin polymerization and depolymerization in a  pH -sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.

Molecular Weight: 19 kDa

Gene ID: 1072, 1073

NCBI Accession: NP_005498, NP_068733

UniProt: P23528, Q9Y281

Pathways: Tube Formation, CXCR4-mediated Signaling Events