Synaptophysin 2 antibody (AA 257-261)

Catalog No. ABIN5689618

Product Details anti-Synaptophysin 2 Antibody

Target: Synaptophysin 2

Binding Specificity: AA 257-261

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Synaptophysin 2 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody detects endogenous levels of total synaptophysin 2 protein.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: synaptophysin 2 antibody was raised against a peptide sequence around aa.257~261 (G-P-T-S-F) derived from Rat synaptophysin 2.

Application Details

Application Notes: Western Blot: 1:500~1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for Synaptophysin 2

Target: Synaptophysin 2

Abstract: Synaptophysin 2 Products

Synonyms: synaptoporin antibody, Synpr antibody

Background: Synaptophysin (SYP) is a neuronal synaptic vesicle glycoprotein that is expressed in neuroendocrine cells and neoplasms, and thus can be used as a marker in tumor diagnosis (1). Synaptophysin contains four transmembrane domains that form a hexameric channel or gap junction-like pore (2). Synaptophysin binds to the SNARE protein synaptobrevin/VAMP, which prevents the inclusion of synaptobrevin in the synaptic vesicle fusion complex and creates a pool of synaptobrevin for exocytosis when synapse activity increases (3). Synaptophysin is also responsible for targeting synaptobrevin 2/VAMP2 to synaptic vesicles, a critical component of the fusion complex (4).

Molecular Weight: 37 kDa

Gene ID: 66030

NCBI Accession: NP_076464

UniProt: P22831