Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Ccl12 antibody

Ccl12 Reactivity: Mouse ELISA, WB, Neut Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN5689163
  • Target See all Ccl12 Antibodies
    Ccl12 (Chemokine (C-C Motif) Ligand 12 (Ccl12))
    Reactivity
    • 20
    • 1
    Mouse
    Host
    • 17
    • 3
    Rabbit
    Clonality
    • 17
    • 3
    Polyclonal
    Conjugate
    • 10
    • 5
    • 2
    • 2
    • 1
    This Ccl12 antibody is un-conjugated
    Application
    • 13
    • 13
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    ELISA, Western Blotting (WB), Neutralization (Neut)
    Purification
    Anti-mMCP-5 specific antibody was purified by affinity chromatography employing immobilized mMCP-5 matrix.
    Immunogen
    Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mMCP-5 (murine MCP-5).
    Top Product
    Discover our top product Ccl12 Primary Antibody
  • Application Notes
    Neutralization:
    To yield one-half maximal inhibition [ND50] of the biological activity of mMCP-5 (100 ng/mL), a concentration of 2.0 - 3.0 μ,g/mL of this antibody is required.

    ELISA:

    To detect mMCP-5 by direct ELISA (using 100 μ,g/mL of this μ,L/well antibody solution) a concentration of at least 0.5 antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mMCP-5.

    Sandwich:

    To detect mMCP-5 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine MCP-5 (XP-5226Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMCP-5.

    Western Blot:

    To detect mMCP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Storage
    -20 °C
    Storage Comment
    MCP-5 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
  • Target
    Ccl12 (Chemokine (C-C Motif) Ligand 12 (Ccl12))
    Alternative Name
    MCP-5 (Ccl12 Products)
    Synonyms
    MCP-5 antibody, Scya12 antibody, chemokine (C-C motif) ligand 12 antibody, Ccl12 antibody
    Background
    MCP-5 is a member of the b-chemokine family (C-C) of cytokines. This chemokine is strongly chemotactic for human monocytes and murine peritoneal macrophages, but is not active on mouse neutrophils and shows only a weak response on mouse eosinophils at high concentrations
    Gene ID
    20293
    UniProt
    Q62401
    Pathways
    Cellular Response to Molecule of Bacterial Origin
You are here:
Support