KC antibody (Biotin)

Catalog No. ABIN5689157

Product Details

Target: KC

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Biotin

Application: Western Blotting (WB), ELISA

Purification: Anti-mKC specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mKC (murine KC).

Application Details

Application Notes: ELISA:

To detect mKC by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mKC.

Sandwich:

To detect mKC by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine KC (XP-5207) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mKC.

Western Blot:

To detect mKC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mKC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: KC antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details

Target: KC

Gene ID: 14825

UniProt: P12850