Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hGCP-2 (100.0 ng/mL), a concentration of 2.0 - 5.0 μ,g/mL of this antibody is required.
ELISA:
To detect hGCP-2 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hGCP-2. br> Sandwich: To detect hGCP-2 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human GCP-2 (XP-5140Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGCP-2.
Western Blot:
To detect hGCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGCP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Restrictions
For Research Use only
Format
Lyophilized
Storage
-20 °C
Storage Comment
GCP-2 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.