FGF acidic antibody
Reactivity: Human
WB, ELISA, Neut
Host: Rabbit
Polyclonal
unconjugated
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- Target
- FGF acidic
- Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Un-conjugated
- Application
- Western Blotting (WB), ELISA, Neutralization (Neut)
- Purification
- Anti-hFGF-acidic specific antibody was purified by affinity chromatography employing immobilized hFGF-acidic matrix.
- Immunogen
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hFGF-acidic (human Fibroblast Growth Factor-acidic).
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anti-FGF acidic antibody
Reactivity: Human WB, ELISA Host: Mouse Monoclonal 3A11G5 unconjugated
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- Application Notes
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Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hFGF-acidic (30.0 ng/mL), a concentration of 0.46 - 0.9 μ,g/mL of this antibody is required.
ELISA:
To detect hFGF-acidic by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hFGF-acidic.
Sandwich: To detect hFGF-acidic by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human FGF-acidic (XP-5136Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-acidic.
Western Blot:
To detect hFGF-acidic by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-acidic is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Storage
- -20 °C
- Storage Comment
- FGF-acidic antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- Target
- FGF acidic
- Alternative Name
- FGF-acidic
- Gene ID
- 2246
- UniProt
- P05230
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