Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Concanavalin A antibody (HRP)

ConA Reactivity: Jack Bean ID, IHC (fro), IF Host: Goat Polyclonal HRP
Catalog No. ABIN568510
  • Target See all Concanavalin A (ConA) Antibodies
    Concanavalin A (ConA)
    Reactivity
    • 6
    • 3
    Jack Bean
    Host
    • 8
    • 1
    Goat
    Clonality
    • 9
    Polyclonal
    Conjugate
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    This Concanavalin A antibody is conjugated to HRP
    Application
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Immunodiffusion (ID), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
    Characteristics
    Molar Ratio: Peroxidase/IgG: ~ 1.3
    Purification
    Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography. Undesired traces of antibody activity are eliminated by Immunoaffinity chromatography
    Immunogen
    The lectin Concanavalin A is a cell-agglutinating protein reacting specifically with molecules which contain a-D-mannopyranosyl, a-D-glucopyranosyl and sterically related residues. At pH 4.5-5.6 it exist as a dimer, above pH 7.0 predominantly as a tetramer. The monomer has a molecular weight of about 52,000. Freund’s complete adjuvant is used in the first step of the immunization procedure.
    Isotype
    IgG
    Top Product
    Discover our top product ConA Primary Antibody
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Reconstitution
    Restore by adding 1 mL sterile di stilled water.
    Concentration
    10.0 mg/mL
    Buffer
    PBS, pH 7.2 withouit preservatives.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    Concanavalin A (ConA)
    Alternative Name
    Concanavalin-A (ConA Products)
    Background
    Concanavalin A (ConA) is a lectin (carbohydrate-binding protein) originally extracted from the jack-bean, Canavalia ensiformis. It is a member of the legume lectin family. It binds specifically to certain structures found in various sugars, glycoproteins, and glycolipids, mainly internal and nonreducing terminal α-D-mannosyl and α-D-glucosyl groups. ConA is a plant mitogen, and is known for its ability to stimulate mouse T-cell subsets giving rise to four functionally distinct T cell populations, including precursors to suppressor T-cell, one subset of human suppressor T-cells as well is sensitive to ConA. ConA was the first lectin to be available on a commercial basis, and is widely used in biology and biochemistry to characterize glycoproteins and other sugar-containing entities on the surface of various cells. It is also used to purify glycosylated macromolecules in lectin affinity chromatography, as well as to study immune regulation by various immune cells.Synonyms: Con-A, ConA, Concanavalin A
    Gene ID
    3823
    UniProt
    P02866
You are here:
Support