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Ficin antibody (Biotin)

Reactivity: Fig IF, WB, DB, EIA, ID, IP, RIA Host: Rabbit Polyclonal Biotin
Catalog No. ABIN568456
  • Target See all Ficin products
    Ficin
    Reactivity
    • 3
    • 2
    Fig
    Host
    • 5
    Rabbit
    Clonality
    • 5
    Polyclonal
    Conjugate
    • 3
    • 2
    This Ficin antibody is conjugated to Biotin
    Application
    • 5
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunofluorescence (IF), Western Blotting (WB), Dot Blot (DB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunoprecipitation (IP), Radioimmunoassay (RIA)
    Characteristics
    Molar Ratio: Biotin/IgG ~ 6.0
    Purification
    Ammonium Sulphate Precipitation and Ion Exchange Chromatography
    Immunogen
    Ficin isolated and purified from fig tree latex. Freund’s complete adjuvant is used in the first step of the immunization procedure
    Isotype
    IgG
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Reconstitution
    Restore by adding 1.0 mL of sterile distilled water
    Concentration
    10.0 mg/mL
    Buffer
    PBS, pH 7.2 without preservatives and foreign proteins
    Preservative
    Without preservative
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
  • Target
    Ficin
    Abstract
    Ficin Products
    Background
    Ficin is classified as a Thiol Protease. It contains a single reactive Cysteine at its active site. The amino acid homology of the active site is similar to that of Papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported Km for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. (3) Ficin is inhibited by Iodoacetamide, Iodoacetic Acid, N-Ethylmaleimide, Mercuric Chloride, DFP (diisopropyl fluorophosphate), TLCK (N-alpha-p-Tosyl-lysine Chloromethyl ketone), and TPCK (N-Tosyl-L-Phenylalanine Chloromethyl Ketone). (1,2) Enzyme Commission (EC) Number: 3.4.22.3 Molecular Weight: 23.8 kDa (1). Extinction Coefficient: E1 % = 21.0 (280 nm) (2)
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