Ficin antibody (Biotin)
Reactivity: Fig
IF, WB, DB, EIA, ID, IP, RIA
Host: Rabbit
Polyclonal
Biotin
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- Target See all Ficin products
- Ficin
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Reactivity
- Fig
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Ficin antibody is conjugated to Biotin
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Application
- Immunofluorescence (IF), Western Blotting (WB), Dot Blot (DB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunoprecipitation (IP), Radioimmunoassay (RIA)
- Characteristics
- Molar Ratio: Biotin/IgG ~ 6.0
- Purification
- Ammonium Sulphate Precipitation and Ion Exchange Chromatography
- Immunogen
- Ficin isolated and purified from fig tree latex. Freund’s complete adjuvant is used in the first step of the immunization procedure
- Isotype
- IgG
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
- Restore by adding 1.0 mL of sterile distilled water
- Concentration
- 10.0 mg/mL
- Buffer
- PBS, pH 7.2 without preservatives and foreign proteins
- Preservative
- Without preservative
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
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- Target
- Ficin
- Abstract
- Ficin Products
- Background
- Ficin is classified as a Thiol Protease. It contains a single reactive Cysteine at its active site. The amino acid homology of the active site is similar to that of Papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported Km for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. (3) Ficin is inhibited by Iodoacetamide, Iodoacetic Acid, N-Ethylmaleimide, Mercuric Chloride, DFP (diisopropyl fluorophosphate), TLCK (N-alpha-p-Tosyl-lysine Chloromethyl ketone), and TPCK (N-Tosyl-L-Phenylalanine Chloromethyl Ketone). (1,2) Enzyme Commission (EC) Number: 3.4.22.3 Molecular Weight: 23.8 kDa (1). Extinction Coefficient: E1 % = 21.0 (280 nm) (2)
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