This Alkaline Phosphatase antibody is conjugated to Biotin
Application
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Radioimmunoassay (RIA), Dot Blot (DB)
Specificity
Alkaline Phosphatase from Escherichia coli. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
Characteristics
Molar Ratio: Biotin/IgG ~ 6.6
Purification
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Immunogen
Alkaline Phosphatase isolated and purified from Escherichia coli. Freund’s complete adjuvant is used in the first step of the immunization procedure.
ALP
Reactivity: Human
Host: Mouse
Monoclonal
3827405
unconjugated
Application Notes
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Format
Liquid
Reconstitution
Restore by adding 1.0 mL of sterile distilled water
Concentration
10.0 mg/mL
Buffer
PBS, pH 7.2 without preservatives and foreign proteins
Preservative
Without preservative
Storage
4 °C/-20 °C
Storage Comment
Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH . Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.Synonyms: JW0374, b0383, phoA