HRP antibody

Catalog No. ABIN568354

Product Details anti-HRP Antibody

Target: HRP (Horseradish Peroxidase (HRP))

Reactivity: Horseradish

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HRP antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunofluorescence (IF), Immunodiffusion (ID), Radioimmunoassay (RIA)

Purification: Solid Phase Affinity Chromatography

Immunogen: Peroxidase isolated and purified from Horseradish. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Restore by adding 0.5 mL of sterile distilled water

Concentration: 2.0 mg/mL

Buffer: PBS, pH 7.2 stabilized with Dextran without preservatives and foreign proteins

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Target Details for HRP

Target: HRP (Horseradish Peroxidase (HRP))

Alternative Name: Horseradish Peroxidase / HRP (HRP Products)

Background: Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.