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Nucleoside Monophosphate Kinase antibody

This Rabbit Polyclonal antibody specifically detects Nucleoside Monophosphate Kinase in WB, IF, DB, EIA, ID, IP and RIA. It exhibits reactivity toward Cow.
Catalog No. ABIN568336

Quick Overview for Nucleoside Monophosphate Kinase antibody (ABIN568336)

Target

Nucleoside Monophosphate Kinase

Reactivity

Cow

Host

  • 3
Rabbit

Clonality

  • 3
Polyclonal

Conjugate

  • 2
  • 1
This Nucleoside Monophosphate Kinase antibody is un-conjugated

Application

  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF), Dot Blot (DB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunoprecipitation (IP), Radioimmunoassay (RIA)
  • Purification

    Ammonium Sulphate Precipitation and Ion Exchange Chromatography

    Immunogen

    Nucleoside Monophosphate Kinase isolated and purified from Bovine liver. Freund’s complete adjuvant is used in the first step of the immunization procedure.

    Isotype

    IgG
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Reconstitution

    Restore by adding 1 mL of sterile distilled water

    Concentration

    10.0 mg/mL

    Buffer

    PBS, pH 7.2 without preservatives and foreign proteins

    Preservative

    Without preservative

    Storage

    4 °C/-20 °C

    Storage Comment

    Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
  • Target

    Nucleoside Monophosphate Kinase

    Background

    Nucleoside monophosphate (NMP) kinases are ubiquitous enzymes involved in the biosynthesis of nucleotides. Each enzyme catalyses the synthesis of a nucleoside diphosphate that is, in turn, converted to a nucleoside triphosphate by a non-specific nucleoside diphosphate kinase. In prokaryotes, there are five NMP kinases, one for the phosphorylation of each NMP, whereas in eukaryotic organisms, the phosphorylation of both uridine monophosphate (UMP) and cytidine monophosphate (CMP) is carried out by a bifunctional UMP/CMP kinase (Liljelund and Lacroute, 1986).Synonyms: NMPK, Nucleoside monophosphokinase
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