LDH5 antibody (Biotin)
LDH5
Reactivity: Rabbit
WB, IF, EIA, DB, ID, IP, RIA
Host: Sheep
Polyclonal
Biotin
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- Target See all LDH5 products
- LDH5 (Lactate Dehydrogenase Isoenzyme 5 (LDH5))
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Reactivity
- Rabbit
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Host
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Sheep
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Clonality
- Polyclonal
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Conjugate
- This LDH5 antibody is conjugated to Biotin
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Application
- Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID), Immunoprecipitation (IP), Radioimmunoassay (RIA)
- Characteristics
- Molar Ratio: Biotin/IgG ~ 6.7
- Purification
- Ammonium Sulphate Precipitation and Ion Exchange Chromatography
- Immunogen
- L-Lactic Dehydrogenase LDH5(M4) isoenzyme, isolated and purified from Rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- Isotype
- IgG
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
- Restore by adding 1.0 mL sterile distilled water
- Concentration
- 10 mg/mL
- Buffer
- PBS, pH 7.2 without preservatives and foreign proteins
- Preservative
- Without preservative
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
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- Target
- LDH5 (Lactate Dehydrogenase Isoenzyme 5 (LDH5))
- Abstract
- LDH5 Products
- Background
- Lactate dehydrogenase (LDH) is present in a wide variety of organisms, including plants and animals. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). There are 5 different isoenzymes of LDH, LDH1 to LDH5, each composed of 4 subunits which may be of 2 different types - M and H subunits. These subunits are encoded by two different genes: The M subunit is encoded by gene LDHA whilst the H subunit is encoded by LDHB. Usually LDH2 is the predominant form in the serum. An LDH1 level higher than the LDH2 level suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH1, into the bloodstream).Synonyms: L-Lactic dehydrogenase LDH-5 Isozyme M4
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