Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID), Immunoprecipitation (IP), Radioimmunoassay (RIA)
Purification
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Immunogen
L-Lactic Dehydrogenase LDH5(M4) isoenzyme, isolated and purified from Rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Reconstitution
Restore by adding 1.0 mL sterile distilled water
Concentration
10 mg/mL
Buffer
PBS, pH 7.2 without preservatives and foreign proteins
Preservative
Without preservative
Storage
4 °C/-20 °C
Storage Comment
Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Lactate dehydrogenase (LDH) is present in a wide variety of organisms, including plants and animals. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). There are 5 different isoenzymes of LDH, LDH1 to LDH5, each composed of 4 subunits which may be of 2 different types - M and H subunits. These subunits are encoded by two different genes: The M subunit is encoded by gene LDHA whilst the H subunit is encoded by LDHB. Usually LDH2 is the predominant form in the serum. An LDH1 level higher than the LDH2 level suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH1, into the bloodstream).Synonyms: L-Lactic dehydrogenase LDH-5 Isozyme M4