HAase antibody (Biotin)
HAase
Reactivity: Sheep
WB, IF, IP, EIA, ID, RIA, DB
Host: Rabbit
Polyclonal
Biotin
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- Target See all HAase Antibodies
- HAase (Hyaluronidase (HAase))
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Reactivity
- Sheep
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This HAase antibody is conjugated to Biotin
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Radioimmunoassay (RIA), Dot Blot (DB)
- Characteristics
- Molar Ratio: Biotin/IgG: ~1.0
- Purification
- Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
- Immunogen
- Hyaluronidase isolated and purified from Sheep testes. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- Isotype
- IgG
- Top Product
- Discover our top product HAase Primary Antibody
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
- Restore by adding 1.0 mL sterile distilled water.
- Concentration
- 10 mg/mL
- Buffer
- PBS, pH 7.2 without preservatives and foreign proteins.
- Preservative
- Without preservative
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
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- Target
- HAase (Hyaluronidase (HAase))
- Alternative Name
- Hyaluronidase (HAase Products)
- Synonyms
- hyaluronidase antibody, Hyaluronidase antibody, hylP antibody, chhy-1 antibody, hylP.1 antibody, hylP.4 antibody
- Background
- Hyaluronidase degrades hyaluronic acid, which is an important structural proteoglycan found in basement membranes and also extracellular matrices. There are six members of the hyaluronidase family. Hyaluronidase PH20 is a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane and plays a role in sperm penetration through the the hyaluronic acid-rich cumulus cell layer surrounding the oocyte. Abnormal expression of this gene has been implicated in degradation of basement membranes leading to tumor invasion and metastasis.Synonyms: HYAL-1, HYAL1, HYAL2, Hyal-2, Hyaluronidase, Hyaluronoglucosaminidase, LUCA-1, LUCA1
- Pathways
- Glycosaminoglycan Metabolic Process
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