Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunodiffusion (ID), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA), Dot Blot (DB)
Specificity
This antibody recognizes Alpha-Glucosidase from Baker's Yeast. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
Purification
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Immunogen
Alpha-Glucosidase isolated and purified from Baker’s Yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Reconstitution
Restore by adding 1.0 mL of sterile distilled water
Concentration
10 mg/mL
Buffer
PBS, pH 7.2 without preservatives and foreign proteins
Preservative
Without preservative
Storage
4 °C/-20 °C
Storage Comment
Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.