Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID), Radioimmunoassay (RIA)
Specificity
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined. Recognizes Aldolase.
Characteristics
Molar Ratio: ~8.1
Purification
Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
Immunogen
Aldolase isolated and purified from Rabbit muscle. Freund's complete adjuvant is used in the first step of the immunization procedure.
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Format
Liquid
Reconstitution
Restore by adding 1.0 mL of sterile distilled water.
Concentration
10.0 mg/mL
Buffer
PBS, pH 7.2 without preservatives and foreign proteins.
Preservative
Without preservative
Storage
4 °C/-20 °C
Storage Comment
Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
ALDA antibody, GSD12 antibody, aldoa antibody, cb79 antibody, sb:cb79 antibody, wu:fa28b10 antibody, wu:fb10b11 antibody, ALDOA antibody, Aldo-1 antibody, Aldo1 antibody, RNALDOG5 antibody, hm:zeh0036 antibody, zgc:77696 antibody, aldolase, fructose-bisphosphate A antibody, aldolase a, fructose-bisphosphate, a antibody, aldolase, fructose-bisphosphate A S homeolog antibody, aldolase A, fructose-bisphosphate antibody, aldolase a, fructose-bisphosphate, b antibody, ALDOA antibody, aldoaa antibody, aldoa antibody, aldoa.S antibody, Aldoa antibody, aldoab antibody
Background
This gene product, Aldolase A (fructose bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3 phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants which encode the same protein.Synonyms: ALDA, Fructose-bisphosphate aldolase A, Lung cancer antigen NY-LU-1, Muscle-type aldolase, NY-LU-1