COL6 antibody

Catalog No. ABIN5680257

Product Details anti-COL6 Antibody

Target: COL6 (Collagen, Type VI (COL6))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Radioimmunoassay (RIA)

Specificity: Human collagen type VI 100 % binding in RIA at 1:10.000 dilution, cross-reactivity with human collagen type I, II, III, IV and V <0.1 %, human elastin and human laminin <0.1 %, human fibronectin < 1 % Human, cross-reactive with bovine

Cross-Reactivity: Chicken, Cow (Bovine), Mouse (Murine), Pig (Porcine), Rat (Rattus)

Cross-Reactivity (Details): Human (cross-reactive with bovine, porcine, chicken, murine and rat tissue)

Characteristics: Collagen type VI is found as 5-10 nm diameter microfibrils (periodicity 100 nm) e.g. in placenta, skin, cartilage and cornea. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Human collagen type VI 100 % , human collagen type I, II, III, IV and V <0.1 %, human elastin and human laminin <0.1 %, human fibronectin < 1 % (RIA at 1:10.000 dilution).

Purification: Affinity purified

Immunogen: Purified collagen type VI from human placenta

Isotype: IgG

Application Details

Application Notes: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg ) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Human placenta,IHC(P), IFA, ELISA, RIA, IB/WB,IHC(P) 60 min at RT or 2-8 °C over night,(purified, lyophilized) 1:80 IFA, 1:500 - 1:1000 IHC(P)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 1 mg/mL

Buffer: phosphate buffered solution, no BSA and preservative added!

Preservative: Without preservative

Storage: -20 °C

Target Details for COL6

Target: COL6 (Collagen, Type VI (COL6))

Alternative Name: Collagen Type VI (COL6 Products)