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COL6 antibody

COL6 Reactivity: Human WB, ELISA, IHC (p), IF, RIA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN5680257
  • Target See all COL6 Antibodies
    COL6 (Collagen, Type VI (COL6))
    Reactivity
    • 31
    • 10
    • 10
    • 6
    • 5
    • 1
    • 1
    • 1
    Human
    Host
    • 21
    • 9
    • 1
    Rabbit
    Clonality
    • 22
    • 9
    Polyclonal
    Conjugate
    • 17
    • 6
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This COL6 antibody is un-conjugated
    Application
    • 20
    • 18
    • 16
    • 8
    • 6
    • 5
    • 5
    • 5
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Radioimmunoassay (RIA)
    Specificity
    Human collagen type VI 100 % binding in RIA at 1:10.000 dilution, cross-reactivity with human collagen type I, II, III, IV and V <0.1 %, human elastin and human laminin <0.1 %, human fibronectin < 1 % Human, cross-reactive with bovine
    Cross-Reactivity
    Chicken, Cow (Bovine), Mouse (Murine), Pig (Porcine), Rat (Rattus)
    Cross-Reactivity (Details)
    Human (cross-reactive with bovine, porcine, chicken, murine and rat tissue)
    Characteristics
    Collagen type VI is found as 5-10 nm diameter microfibrils (periodicity 100 nm) e.g. in placenta, skin, cartilage and cornea. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Human collagen type VI 100 % , human collagen type I, II, III, IV and V <0.1 %, human elastin and human laminin <0.1 %, human fibronectin < 1 % (RIA at 1:10.000 dilution).
    Purification
    Affinity purified
    Immunogen
    Purified collagen type VI from human placenta
    Isotype
    IgG
    Top Product
    Discover our top product COL6 Primary Antibody
  • Application Notes
    After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg ) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Human placenta,IHC(P), IFA, ELISA, RIA, IB/WB,IHC(P) 60 min at RT or 2-8 °C over night,(purified, lyophilized) 1:80 IFA, 1:500 - 1:1000 IHC(P)
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Concentration
    1 mg/mL
    Buffer
    phosphate buffered solution, no BSA and preservative added!
    Preservative
    Without preservative
    Storage
    -20 °C
  • Target
    COL6 (Collagen, Type VI (COL6))
    Alternative Name
    Collagen Type VI (COL6 Products)
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