MAD2L1 antibody

Catalog No. ABIN567786

Product Details anti-MAD2L1 Antibody

Target: MAD2L1 (MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAD2L1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Specificity: This antibody reacts with MAD2 (25 kDa) on Western blotting and Immunoprecipitation.

Purification: Protein-A Agarose Chromatography of hybridoma supernatant.

Immunogen: Recombinant Human MAD2.

Clone: 2G9

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 50 % Glycerol without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for MAD2L1

Target: MAD2L1 (MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1))

Alternative Name: MAD2L1 (MAD2L1 Products)

Background: Some of the spindle checkpoint components are evolutionarily conserved from yeast to human. They include mitotic arrest deficiency 1 (MAD1), MAD2, MAD3/BUBR1, budding uninhibited by benomyl 1 (BUB1), BUB3, and monopolar spindle 1 (MPS1). MAD1 interacts with several checkpoint proteins, forming a complex with MAD2 and recruiting MAD2 to unattached kinetochores. MAD1 and Cdc20 can bind MAD2, but these two bindings are exclusive. They share a 10-residue MAD2-binding motif. It has been suggested that MAD1 acts as a positive regulator and a competitive inhibitor of the MAD2-Cdc20 complex.Synonyms: MAD2, Mitotic spindle assembly checkpoint protein MAD2A

Gene ID: 4085

NCBI Accession: NP_002349

UniProt: Q13257