SMAD5 antibody (pSer463, pSer465)

Catalog No. ABIN5675103

Product Details anti-SMAD5 Antibody

Target: SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))

Binding Specificity: pSer463, pSer465

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Sheep,Pig,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthesised phosphopeptide derived from human SMAD5 around the phosphorylation site of Ser463 + Ser465

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for SMAD5

Target: SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))

Alternative Name: SMAD5 ( (SMAD5 Products)

Synonyms: DWFC antibody, JV5-1 antibody, MADH5 antibody, Madh5 antibody, SBN antibody, fb67b04 antibody, mad antibody, madh5 antibody, sb:cb400 antibody, smd5 antibody, wu:fb67b04 antibody, SMAD5 antibody, DKFZp468M0619 antibody, 1110051M15Rik antibody, AI451355 antibody, Dwf-C antibody, MusMLP antibody, SMAD family member 5 antibody, SMAD5 antibody, Smad5 antibody, smad5 antibody

Background:

Synonyms: Mothers against decapentaplegic homolog 5, SMAD5, MAD homolog 5, Mothers against DPP homolog 5, JV5-1, SMAD family member 5, SMAD 5, Smad5, hSmad5

Background: Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).

Gene ID: 4090

UniProt: Q99717