CERS6 antibody (AA 62-131)

Catalog No. ABIN566979

Product Details anti-CERS6 Antibody

Target: CERS6 (Ceramide Synthase 6 (CERS6))

Binding Specificity: AA 62-131

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CERS6 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Mouse monoclonal antibody raised against a partial recombinant LASS6.

Sequence: PCAIALNIQA NGPQIAPPNA ILEKVFTAIT KHPDEKRLEG LSKQLDWDVR SIQRWFRQRR NQEKPSTLTR

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: LASS6 (NP_982288, 62 a.a. ~ 131 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone: 5H7

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-CERS6 antibody (ABIN566979)

Suzuki, Cao, Kato, Komizu, Mizutani, Tanaka, Arima, Tai, Yanagisawa, Togawa, Shiraishi, Usami, Taniguchi, Fukui, Yokoi, Wakahara, Hasegawa, Mizutani, Igarashi, Inokuchi, Iwaki, Fujii, Satou et al.: "Targeting ceramide synthase 6-dependent metastasis-prone phenotype in lung cancer cells. ..." in: The Journal of clinical investigation, Vol. 126, Issue 1, pp. 254-65, (2016) (PubMed).

Gerl, Bittl, Kirchner, Sachsenheimer, Brunner, Lüchtenborg, Özbalci, Wiedemann, Wegehingel, Nickel, Haberkant, Schultz, Krüger, Brügger: "Sphingosine-1-Phosphate Lyase Deficient Cells as a Tool to Study Protein Lipid Interactions." in: PLoS ONE, Vol. 11, Issue 4, pp. e0153009, (2016) (PubMed).

Jensen, Calvert, Volpert, Kouri, Hurley, Luciano, Wu, Chalastanis, Futerman, Stegh: "Bcl2L13 is a ceramide synthase inhibitor in glioblastoma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 15, pp. 5682-7, (2014) (PubMed).

Laviad, Kelly, Merrill, Futerman: "Modulation of ceramide synthase activity via dimerization." in: The Journal of biological chemistry, Vol. 287, Issue 25, pp. 21025-33, (2012) (PubMed).

Yamane, Miyazawa, Moriya, Abe, Yamane et al.: "D,L-Threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol (DL-PDMP) increases endoplasmic reticulum stress, autophagy and apoptosis accompanying ceramide accumulation via ceramide synthase 5 protein ..." in: Biochimie, Vol. 93, Issue 9, pp. 1446-59, (2011) (PubMed).

Mesicek, Lee, Feldman, Jiang, Skobeleva, Berdyshev, Haimovitz-Friedman, Fuks, Kolesnick: "Ceramide synthases 2, 5, and 6 confer distinct roles in radiation-induced apoptosis in HeLa cells." in: Cellular signalling, Vol. 22, Issue 9, pp. 1300-7, (2010) (PubMed).

in: (PubMed).

Target Details for CERS6

Target: CERS6 (Ceramide Synthase 6 (CERS6))

Alternative Name: LASS6 (CERS6 Products)

Background: Full Gene Name: LAG1 homolog, ceramide synthase 6
Synonyms: CerS6,MGC129949,MGC129950

Gene ID: 253782

NCBI Accession: NM_203463