PIKFYVE antibody (AA 342-451)

Catalog No. ABIN566937

Product Details anti-PIKFYVE Antibody

Target: PIKFYVE (phosphoinositide Kinase, FYVE Finger Containing (PIKFYVE))

Binding Specificity: AA 342-451

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PIKFYVE antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), RNA Interference (RNAi)

Purpose: Mouse monoclonal antibody raised against a partial recombinant PIP5K3.

Sequence: LQSTEFSETP SPDSDSVNSV EGHSEPSWFK DIKFDDSDTE QIAEEGDDNL ANSASPSKRT SVSSFQSTVD SDSAASISLN VELDNVNFHI KKPSKYPHVP PHPADQKGRR

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: PIP5K3 (NP_689884, 342 a.a. ~ 451 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone: 6C7

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-PIKFYVE antibody (ABIN566937)

Takasuga, Horie, Sasaki, Sun-Wada, Kawamura, Iizuka, Mizuno, Eguchi, Kofuji, Kimura, Yamazaki, Horie, Odanaga, Sato, Chida, Kontani, Harada, Katada, Suzuki, Wada, Ohnishi, Sasaki: "Critical roles of type III phosphatidylinositol phosphate kinase in murine embryonic visceral endoderm and adult intestine." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 5, pp. 1726-31, (2013) (PubMed).

Tsuruta, Green, Rousset, Dolmetsch: "PIKfyve regulates CaV1.2 degradation and prevents excitotoxic cell death." in: The Journal of cell biology, Vol. 187, Issue 2, pp. 279-94, (2009) (PubMed).

Target Details for PIKFYVE

Target: PIKFYVE (phosphoinositide Kinase, FYVE Finger Containing (PIKFYVE))

Alternative Name: PIP5K3 (PIKFYVE Products)

Background: Full Gene Name: phosphatidylinositol-3-phosphate/phosphatidylinositol 5-kinase, type III
Synonyms: CFD,FAB1,KIAA0981,MGC40423,PIKFYVE,PIP5K

Gene ID: 200576

NCBI Accession: NM_152671

Pathways: Inositol Metabolic Process