Shugoshin antibody (AA 1-292)

Catalog No. ABIN566873

Product Details anti-Shugoshin Antibody

Target: Shugoshin (SGOL1) (Shugoshin 1 (SGOL1))

Binding Specificity: AA 1-292

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Shugoshin antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Purpose: Mouse monoclonal antibody raised against a full length recombinant SGOL1.

Sequence: MAKERCLKKS FQDSLEDIKK RMKEKRNKNL AEIGKRRSFI AAPCQIITNT STLLKNYQDN NKMLVLALEN EKSKVKEAQD IILQLRKECY YLTCQLYALK GKLTSQQTVE PAQNQEICSS GMDPNSDDSS RNLFVKDLPQ IPLEETELPG QGESFQIEAT PPETQQSPHL SLKDITNVSL YPVVKIRRLS LSPKKNKASP AVALPKRRCT ASVNYKEPTL ASKLRRGDPF TDLCFLNSPI FKQKKDLRRS KKRALEVSPA KEAIFILYYV REFVSRFPDC RKCKLETHIC LR

Cross-Reactivity: Human, Rat

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: SGOL1 (AAH17867, 1 a.a. ~ 292 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone: 3C11

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-Shugoshin antibody (ABIN566873)

Nijenhuis, Vallardi, Teixeira, Kops, Saurin: "Negative feedback at kinetochores underlies a responsive spindle checkpoint signal." in: Nature cell biology, Vol. 16, Issue 12, pp. 1257-64, (2014) (PubMed).

Wang, Mayer, Stemmann, Nigg: "Centromere DNA decatenation depends on cohesin removal and is required for mammalian cell division." in: Journal of cell science, Vol. 123, Issue Pt 5, pp. 806-13, (2010) (PubMed).

Shouse, Nobumori, Liu: "A B56gamma mutation in lung cancer disrupts the p53-dependent tumor-suppressor function of protein phosphatase 2A." in: Oncogene, Vol. 29, Issue 27, pp. 3933-41, (2010) (PubMed).

Nozawa, Nagao, Masuda, Iwasaki, Hirota, Nozaki, Kimura, Obuse: "Human POGZ modulates dissociation of HP1alpha from mitotic chromosome arms through Aurora B activation." in: Nature cell biology, Vol. 12, Issue 7, pp. 719-27, (2010) (PubMed).

Garcia-Cruz, Brieño, Roig, Grossmann, Velilla, Pujol, Cabero, Pessarrodona, Barbero, Garcia Caldés: "Dynamics of cohesin proteins REC8, STAG3, SMC1 beta and SMC3 are consistent with a role in sister chromatid cohesion during meiosis in human oocytes." in: Human reproduction (Oxford, England), Vol. 25, Issue 9, pp. 2316-27, (2010) (PubMed).

Hayashi-Takanaka, Yamagata, Nozaki, Kimura: "Visualizing histone modifications in living cells: spatiotemporal dynamics of H3 phosphorylation during interphase." in: The Journal of cell biology, Vol. 187, Issue 6, pp. 781-90, (2009) (PubMed).

Fu, Ding, Yuan, Aikhionbare, Yao, Cai, Jiang, Yao: "Phosphorylation of human Sgo1 by NEK2A is essential for chromosome congression in mitosis." in: Cell research, Vol. 17, Issue 7, pp. 608-18, (2007) (PubMed).

Thein, Kleylein-Sohn, Nigg, Gruneberg: "Astrin is required for the maintenance of sister chromatid cohesion and centrosome integrity." in: The Journal of cell biology, Vol. 178, Issue 3, pp. 345-54, (2007) (PubMed).

Dai, Sullivan, Higgins: "Regulation of mitotic chromosome cohesion by Haspin and Aurora B." in: Developmental cell, Vol. 11, Issue 5, pp. 741-50, (2006) (PubMed).

Target Details for Shugoshin

Target: Shugoshin (SGOL1) (Shugoshin 1 (SGOL1))

Alternative Name: SGOL1 (SGOL1 Products)

Synonyms: sgo1 antibody, NY-BR-85 antibody, SGO antibody, Sgo1 antibody, 3300001M08Rik antibody, C81037 antibody, shugoshin 1 antibody, shugoshin centromeric cohesion 1 antibody, shugoshin 1 L homeolog antibody, Sgo1 antibody, sgo1 antibody, sgo1.L antibody, SGO1 antibody

Background: Full Gene Name: shugoshin-like 1 (S. pombe)
Synonyms: NY-BR-85,SGO,Sgo1

Gene ID: 151648

Pathways: Maintenance of Protein Location