Recombinant AOC3 antibody

Catalog No. ABIN5668213

Product Details anti-AOC3 Antibody

Target: AOC3 (Amine Oxidase, Copper Containing 3 (Vascular Adhesion Protein 1) (AOC3))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This AOC3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Inhibition (Inh), Immunoaffinity Chromatography (IAC)

Purpose: Anti-VAP-1 [TK8-14], Rabbit IgG, kappa

Specificity: This antibody is specific for human VAP-1, a glycosylated homodimeric membrane protein consisting of two 90 kDa subunits connected by disulfide bonds. The recognized epitope is present both in dimeric and monomeric forms of VAP-1.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing specific pathogen-free BALB/c mice with affinity-purified VAP-1 obtained from tonsil stromal lysate.

Clone: TK8-14

Isotype: IgG kappa

Application Details

Application Notes: The original version of this antibody (mouse IgG2a) was generated, and its specificity was characterized by reciprocal immunoprecipitation with another anti-VAP-1 antibody (1B2). In gel analyses, this antibody was shown to react with the dimeric 170- kDa VAP-1 and, under reducing conditions, with the monomeric 90- kDa form of VAP-1. Furthermore, it was used for both immunofluorescence staining and flow cytometry analysis to assess the expression of VAP-1 in rat HEV-like endothelial cell line Ax transfectants (Salmi et al., 1998, PMID: 9605169). The original version of this antibody was biotinylated and characterized via various assays. In an ELISA, it was used to detect serum VAP-1 levels. In a Western blot, it was employed to analyze VAP-1 presence and molecular weight. In immunoaffinity chromatography, it was used to purify VAP-1 from serum samples. In an adhesion assay, it was used to specifically deplete sVAP-1 from serum (Kurkijärvi et al., 1998, PMID: 9686623). This antibody was used in transwell migration assays to study the phenotype of transmigrated monocytes by flow cytometry, it was employed to pretreat primary human hepatic sinusoidal endothelial cells to study its effect on monocyte transmigration. The results showed a significant reduction in monocyte transmigration upon pretreatment, indicating the antibody's role as an inhibitor of VAP-1 function (Zimmermann et al., 2012, DOI: 10.1136/gutjnl-2012-302514b.126). This antibody was used in flow cytometry and immunocytochemistry to detect and characterize human hematopoietic stem cells based on VAP-1 expression. In flow cytometry, this antibody was utilized to stain fresh human bone marrow and cord blood (CB) cells. In an immunocytochemistry assay, it was used to stain FACS-sorted CD34⁺ CB cells for visualization under a microscope. Furthermore, TK8-14 was utilized in RNAseq analysis to investigate gene expression profiles of VAP-1⁺ and VAP-1^- HSCs (Iftakhar-E-Khuda et al., 2021, PMID: 34719737).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for AOC3

Target: AOC3 (Amine Oxidase, Copper Containing 3 (Vascular Adhesion Protein 1) (AOC3))

Alternative Name: VAP-1 (AOC3 Products)

Background: Vascular adhesion protein 1, VAP1, Copper amine oxidase, HPAO, Membrane primary amine oxidase, Vascular adhesion protein 1, Amine oxidase [copper-containing] 3, Amine oxidase copper-containing 3, Copper amine oxidase, HPAO, Semicarbazide-sensitive amine oxidase 1, Semicarbazide-sensitive, AOC3, Amine oxidase, copper containing 3, amine oxidase, amine oxidase, Vascular adhesion protein 1, SSAO, copper-containing amine:oxy, EC:1.4.3.21

UniProt: Q16853

Pathways: Feeding Behaviour