Recombinant RTN4RL2 antibody (AA 279-420)

Catalog No. ABIN5668205

Product Details anti-RTN4RL2 Antibody

Target: RTN4RL2 (Reticulon 4 Receptor-Like 2 (RTN4RL2))

Antibody Type: Recombinant Antibody

Binding Specificity: AA 279-420

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This RTN4RL2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP)

Purpose: Anti-NgR2 [P14], Rabbit IgG, kappa

Specificity: The antibody binds to human NgR2 with a Kd of 2.3 nM and to rat NgR2 with a Kd of 0.48 nM (bivalent binding). The antibody binds specifically to residues 351-399 of NgR2, a member of the Nogo-66 receptor family which inhibits axonal growth and sporulating in the CNS.

Cross-Reactivity: Human, Rat

Characteristics:

Original Species of Ab: Rabbit

Original Format of Ab: IgG

Purification: Protein A affinity purified

Immunogen: (His)6-tagged fragment of rat NgR2 (amino acids 279-420).

Clone: P14

Isotype: IgG kappa

Application Details

Application Notes: The original antibody was used for for an ELISA on the NgR2 antigen to confirm the binding of the antibody. This antibody was used for a western blot on lysates of 293F cells transfected with vectors for mammalian cell expression of human NgR1, NgR2, and NgR3. This antibody was used for flow cytometry on 293F cells transfected with human NgR1, NgR2, and NgR3 mammalian cell expression vectors. This antibody was used for surface plasmon resonance on NgR1 and NgR2 to determine its binding affinity for its antigen. The antibody was used for immunofluorescence on PC12 cells and PC12-NgR1 or PC12-NgR2. This antibody was used for immunoprecipitation on rat PC12 cell lines stably expressing full-length rat NgR1 or NgR2 (Hofer et al., 2008, PMID:17140598).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for RTN4RL2

Target: RTN4RL2 (Reticulon 4 Receptor-Like 2 (RTN4RL2))

Alternative Name: NgR2 (RTN4RL2 Products)

Background: Nogo-66 Receptor

UniProt: Q86UN3