Recombinant CD32/CD16 antibody

Catalog No. ABIN5668180

Product Details anti-CD32/CD16 Antibody

Target: CD32/CD16

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD32/CD16 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Blocking Reagent (BR), Immunohistochemistry (IHC)

Specificity: This antibody binds to an epitope on the extracellular domains of the mouse FcγIII and FcγII, thereby inhibiting their ability to bind antibodies via their Fc region. receptors. Also binding the FcγI receptor (CD64) via its Fc domain.

Characteristics:

OriginalSpeciesName: Rat

OriginalFormat: IgG2b

Purification: Purified antibody.

Immunogen: Mouse BALB/c Macrophage J774 Cell Line.

Clone: 2-4G2

Isotype: IgG2b kappa

Application Details

Application Notes: This antibody binds murine Fc Receptors CD16/CD32 and inhibits binding of antibodies to these. It can therefore be used as an Fc-blocking reagent, to reduce non-specific staining of cells by antibodies binding via their Fc-region. A similar effect can be achieved using the Fc Silent range of antibodies made by Absolute Antibody that prevent these interactions at the level of the Fc receptor binding site, which has been mutated to lack affinity for the Fc receptors.

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD32/CD16

Target: CD32/CD16

Alternative Name: CD16/CD32 (CD32/CD16 Products)

Background: Fcγ III/II Receptor, Low affinity immunoglobulin gamma Fc region receptor III, IgG Fc receptor III, Fc-gamma RIII, FcRIII, CD16, Low affinity immunoglobulin gamma Fc region receptor II, Fc gamma receptor IIB, Fc-gamma RII, Fc-gamma-RIIB, FcRII IgG Fc rec

UniProt: P08508, P08101