Recombinant DDDDK Tag antibody

Catalog No. ABIN5668123

Product Details anti-DDDDK Tag Antibody

Target: DDDDK Tag

Antibody Type: Recombinant Antibody

Reactivity: Please inquire

Host: Rabbit

Clonality: Chimeric

Conjugate: This DDDDK Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Specificity: This antibody recognises the DYKDDDDK-tag, a widely used epitope tag.

Characteristics:

OriginalSpeciesName: Mouse

OriginalFormat: IgG1

Purification: Purified antibody.

Immunogen: Interleukin 2 genetically fused to the peptided DYKDDDDK, corresponding to the DDDDK epitope tag.

Clone: M2-1

Isotype: IgG kappa

Application Details

Application Notes: This antibody recognises he widely used DDDDK-tag commonly used as an epitope tag in fusion proteins. The tag can be recognised at any position within a fusion protein, i.e. N-terminally, Met-N-terminally, C-terminally as well as internally. Binding of this antivbody is not sensitive to calcium. This antibody recognises both native and denatured protein.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for DDDDK Tag

Target: DDDDK Tag

Alternative Name: DDDDK-tag (DDDDK Tag Products)

Target Type: Tag

Background: FLAG-tag, Equivalent to FLAG antibodies from Sigma, DYKDDDDK, DDDK, AspTyrLysAspAspAspAspLy