Recombinant Fluorescein antibody

Catalog No. ABIN5668039

Product Details

Target: Fluorescein

Antibody Type: Recombinant Antibody

Host: Rat

Clonality: Chimeric

Conjugate: Un-conjugated

Application: In vivo Studies (in vivo), Negative Control (NC)

Purpose: Anti-Fluorescein [4-4-20 (enhanced)], Rat IgG2a, kappa

Specificity: This antibody is specific for fluorescein I.

Cross-Reactivity: Various Species

Characteristics:

Original Species of Ab: Human

Original Format of Ab: scFv

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BALB/c mice with fluorescein I-keyhole limpet hemocyanin (FlI-KLH).

Clone: 4-4-20 (enhanced)

Isotype: IgG2a kappa

Application Details

Application Notes: This antibody, a humanized, enhanced scFv of the original mouse IgG2a antibody, was constructed by grafting the CDRs of the original antibody onto the humanized 4D5 anti-HER2 antibody framework, and subsequently enhancing it with respect to folding and stability via randomization in the form of DNA shuffling, as well as oligonucleotides encoding mutations at defined positions (Jung et al., 1999, PMID: 10556036). The original antibody was tested against FlI-BSA. The enhanced antibody fragment was tested against FITC-BSA. This antibody can be used as an isotype control in both in vitro and in vivo applications. This antibody is not conjugated to fluorescein.

Comment:

This is a chimeric antibody created as part of a panel offering antibodies of the same specificity in different formats (species, isotype, subtype and modified versions) for use as isotype controls.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details

Target: Fluorescein

Target Type: Chemical

Background: FL, FlI, FITC, Fluorescein isothiocyanate, fluorescein isothiocyanate, isomer 1, Fluorescyl, Resorcinolphthalein, C.I. 45350, solvent yellow 94, D & C yellow no. 7, Angiofluor, Japan yellow 201, Soap yellow, 3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one