CHFR antibody (AA 1-652)

Catalog No. ABIN565913

Product Details anti-CHFR Antibody

Target: CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))

Binding Specificity: AA 1-652

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CHFR antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), RNA Interference (RNAi)

Purpose: Mouse monoclonal antibody raised against a full length recombinant CHFR.

Sequence: MERPEEGKQS PPPQPWGRLL RLGAEEGEPH VLLRKREWTI GRRRGCDLSF PSNKLVSGDH CRIVVDEKSG QVTLEDTSTS GTVINKLKVV KKQTCPLQTG DVIYLVYRKN EPEHNVAYLY ESLSEKQGMT QESFDTSGAG AGRGADPRVP PSSPATQVCF EEPQPSTSTS DLFPTASASS TEPSPAGRER SSSCGSGGGG ISPKGSGPSV ASDEVSSFAS ALPDRKTASF SSLEPQDQED LEPVKKKMRG DGDLDLNGQL LVAQPRRNAQ TVHEDVRAAA GKPDKMEETL TCIICQDLLH DCVSLQPCMH TFCAACYSGW MERSSLCPTC RCPVERICKN HILNNLVEAY LIQHPDKSRS EEDVQSMDAR NKITQDMLQP KVRRSFSDEE GSSEDLLELS DVDSESSDIS QPYVVCRQCP EYRRQAAQPP HCPAPEGEPG APQALGDAPS TSVSLTTAVQ DYVCPLQGSH ALCTCCFQPM PDRRVEREQD PRVAPQQCAV CLQPFCHLYW GCTRTGCYGC LAPFCELNLG DKCLDGVLNN NSYESDILKN YLATRGLTWK NMLTESLVAL QRGVFLLSDY RVTGDTVLCY CCGLRSFREL TYQYRQNIPA SELPVAVTSR PDCYWGRNCR TQVKAHHAMK FNHICEQTRF KN

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: CHFR (AAH12072.1, 1 a.a. ~ 652 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone: 1H3-A12

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-CHFR antibody (ABIN565913)

Fujita, Yamamoto, Imamura, Nakamura, Maehara, Tsuneyoshi, Oda: "Expression level of the mitotic checkpoint protein and G2-M cell cycle regulators and prognosis in gastrointestinal stromal tumors in the stomach." in: Virchows Archiv : an international journal of pathology, Vol. 460, Issue 2, pp. 163-9, (2012) (PubMed).

Kashima, Idogawa, Mita, Shitashige, Yamada, Ogi, Suzuki, Toyota, Ariga, Sasaki, Tokino: "CHFR protein regulates mitotic checkpoint by targeting PARP-1 protein for ubiquitination and degradation." in: The Journal of biological chemistry, Vol. 287, Issue 16, pp. 12975-84, (2012) (PubMed).

Hu, Huang, Sun, Wu, Xu, Yin, Chen, Jiang, Shen: "Pathobiologic implications of methylation and expression status of Runx3 and CHFR genes in gastric cancer." in: Medical oncology (Northwood, London, England), Vol. 28, Issue 2, pp. 447-54, (2011) (PubMed).

Privette, Weier, Nguyen, Yu, Petty: "Loss of CHFR in human mammary epithelial cells causes genomic instability by disrupting the mitotic spindle assembly checkpoint." in: Neoplasia (New York, N.Y.), Vol. 10, Issue 7, pp. 643-52, (2008) (PubMed).

Gao, Xin, Zhang, Zhou: "Mechanism and pathobiologic implications of CHFR promoter methylation in gastric carcinoma." in: World journal of gastroenterology : WJG, Vol. 14, Issue 32, pp. 5000-7, (2008) (PubMed).

Milne, Sitarz, Carvalho, Polak, Ligtenberg, Pauwels, Offerhaus, Weterman: "Molecular analysis of primary gastric cancer, corresponding xenografts, and 2 novel gastric carcinoma cell lines reveals novel alterations in gastric carcinogenesis." in: Human pathology, Vol. 38, Issue 6, pp. 903-13, (2007) (PubMed).

Privette, González, Ding, Kleer, Petty: "Altered expression of the early mitotic checkpoint protein, CHFR, in breast cancers: implications for tumor suppression." in: Cancer research, Vol. 67, Issue 13, pp. 6064-74, (2007) (PubMed).

Target Details for CHFR

Target: CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))

Alternative Name: CHFR (CHFR Products)

Synonyms: RNF116 antibody, RNF196 antibody, CHFR antibody, fc43f10 antibody, si:dkey-69h6.7 antibody, wu:fc43f10 antibody, rnf116 antibody, rnf196 antibody, 5730484M20Rik antibody, C230082M18 antibody, checkpoint with forkhead and ring finger domains antibody, checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase antibody, checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase L homeolog antibody, CHFR antibody, chfr antibody, Chfr antibody, chfr.L antibody

Background: Full Gene Name: checkpoint with forkhead and ring finger domains
Synonyms: FLJ10796,FLJ33629,RNF116,RNF196

Gene ID: 55743