GAPDHS antibody (AA 1-408)

Catalog No. ABIN565269

Product Details anti-GAPDHS Antibody

Target: GAPDHS (Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS))

Binding Specificity: AA 1-408

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAPDHS antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Mouse monoclonal antibody raised against a full length recombinant GAPDS.

Sequence: MSKRDIVLTN VTVVQLLRQP CPVTRAPPPP EPKAEVEPQP QPEPTPVREE IKPPPPPLPP HPATPPPKMV SVARELTVGI NGFGRIGRLV LRACMEKGVK VVAVNDPFID PEYMVYMFKY DSTHGRYKGS VEFRNGQLVV DNHEISVYQC KEPKQIPWRA VGSPYVVEST GVYLSIQAAS DHISAGAQRV VISAPSPDAP MFVMGVNEND YNPGSMNIVS NASCTTNCLA PLAKVIHERF GIVEGLMTTV HSYTATQKTV DGPSRKAWRD GRGAHQNIIP ASTGAAKAVT KVIPELKGKL TGMAFRVPTP DVSVVDLTCR LAQPAPYSAI KEAVKAAAKG PMAGILAYTE DEVVSTDFLG DTHSSIFDAK AGIALNDNFV KLISWYDNEY GYSHRVVDLL RYMFSRDK

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: GAPDS (AAH36373, 1 a.a. ~ 408 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone: 2E3-2E10

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-GAPDHS antibody (ABIN565269)

Kim, Hata, Kang: "Down-regulation of miR-96 by bone morphogenetic protein signaling is critical for vascular smooth muscle cell phenotype modulation." in: Journal of cellular biochemistry, Vol. 115, Issue 5, pp. 889-95, (2014) (PubMed).

Kang, Davis-Dusenbery, Nguyen, Lal, Lieberman, Van Aelst, Lagna, Hata: "Bone morphogenetic protein 4 promotes vascular smooth muscle contractility by activating microRNA-21 (miR-21), which down-regulates expression of family of dedicator of cytokinesis (DOCK) proteins." in: The Journal of biological chemistry, Vol. 287, Issue 6, pp. 3976-86, (2012) (PubMed).

Kuravsky, Schmalhausen, Pozdnyakova, Muronetz: "Isolation of antibodies against different protein conformations using immunoaffinity chromatography." in: Analytical biochemistry, Vol. 426, Issue 1, pp. 47-53, (2012) (PubMed).

Kang, Louie, Weisman, Sheu-Gruttadauria, Davis-Dusenbery, Lagna, Hata: "Inhibition of microRNA-302 (miR-302) by bone morphogenetic protein 4 (BMP4) facilitates the BMP signaling pathway." in: The Journal of biological chemistry, Vol. 287, Issue 46, pp. 38656-64, (2012) (PubMed).

Chan, Weisman, Kang, Nguyen, Hickman, Mecker, Hill, Lagna, Hata: "The amiloride derivative phenamil attenuates pulmonary vascular remodeling by activating NFAT and the bone morphogenetic protein signaling pathway." in: Molecular and cellular biology, Vol. 31, Issue 3, pp. 517-30, (2011) (PubMed).

Davis-Dusenbery, Chan, Reno, Weisman, Layne, Lagna, Hata et al.: "down-regulation of Kruppel-like factor-4 (KLF4) by microRNA-143/145 is critical for modulation of vascular smooth muscle cell phenotype by transforming growth factor-beta and bone morphogenetic ..." in: The Journal of biological chemistry, Vol. 286, Issue 32, pp. 28097-110, (2011) (PubMed).

Chan, Hilyard, Wu, Davis, Hill, Lal, Lieberman, Lagna, Hata: "Molecular basis for antagonism between PDGF and the TGFbeta family of signalling pathways by control of miR-24 expression." in: The EMBO journal, Vol. 29, Issue 3, pp. 559-73, (2010) (PubMed).

Davis, Hilyard, Nguyen, Lagna, Hata: "Smad proteins bind a conserved RNA sequence to promote microRNA maturation by Drosha." in: Molecular cell, Vol. 39, Issue 3, pp. 373-84, (2010) (PubMed).

Elkina, Kuravsky, Eldarov, Stogov, Muronetz, Schmalhausen: "Recombinant human sperm-specific glyceraldehyde-3-phosphate dehydrogenase: structural basis for enhanced stability." in: Biochimica et biophysica acta, Vol. 1804, Issue 12, pp. 2207-12, (2010) (PubMed).

Davis, Hilyard, Nguyen, Lagna, Hata: "Induction of microRNA-221 by platelet-derived growth factor signaling is critical for modulation of vascular smooth muscle phenotype." in: The Journal of biological chemistry, Vol. 284, Issue 6, pp. 3728-38, (2009) (PubMed).

Shchutskaya, Elkina, Kuravsky, Bragina, Schmalhausen: "Investigation of glyceraldehyde-3-phosphate dehydrogenase from human sperms." in: Biochemistry. Biokhimii?a, Vol. 73, Issue 2, pp. 185-91, (2008) (PubMed).

Davis, Hilyard, Lagna, Hata: "SMAD proteins control DROSHA-mediated microRNA maturation." in: Nature, Vol. 454, Issue 7200, pp. 56-61, (2008) (PubMed).

Target Details for GAPDHS

Target: GAPDHS (Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS))

Alternative Name: GAPDHS (GAPDHS Products)

Synonyms: GAPDS antibody, GAPDHS antibody, GAPD2 antibody, GAPDH-2 antibody, HSD-35 antibody, Gapd-s antibody, Gapds antibody, gapdh-2 antibody, cb350 antibody, fb71f08 antibody, fk58c09 antibody, g3pdh antibody, gapdh antibody, gapds antibody, wu:fb71f08 antibody, wu:fk58c09 antibody, zgc:76908 antibody, glyceraldehyde-3-phosphate dehydrogenase, spermatogenic antibody, GAPDHS antibody, Gapdhs antibody, gapdhs antibody

Background: Full Gene Name: glyceraldehyde-3-phosphate dehydrogenase, spermatogenic
Synonyms: GAPD2,GAPDH-2,GAPDS,HSD-35

Gene ID: 26330

Pathways: Regulation of Carbohydrate Metabolic Process