POLI antibody (AA 616-715)
(3 references)-
- Target See all POLI Antibodies
- POLI (Polymerase (DNA Directed) iota (POLI))
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Binding Specificity
- AA 616-715
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This POLI antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Purpose
- Mouse monoclonal antibody raised against a partial recombinant POLI.
- Sequence
- PQGFHFTNSN PAVSAFHSFP NLQSEQLFSR NHTTDSHKQT VATDSHEGLT ENREPDSVDE KITFPSDIDP QVFYELPEAV QKELLAEWKR AGSDFHIGHK
- Cross-Reactivity
- Human
- Characteristics
- Antibody Reactive Against Recombinant Protein.
- Immunogen
- POLI (AAH32662, 616 a.a. ~ 715 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Clone
- 8G9
- Isotype
- IgG3
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anti-Polymerase (DNA Directed) iota (POLI) (N-Term) antibody
POLI Reactivity: Human, Rat, Mouse WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Polymerase (DNA Directed) iota (POLI) (AA 458-711) antibodyPOLI Reactivity: Human ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Polymerase (DNA Directed) iota (POLI) (N-Term) antibodyPOLI Reactivity: Human WB, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Polymerase (DNA Directed) iota (POLI) (AA 458-740) antibodyPOLI Reactivity: Human WB, IHC, IHC (p) Host: Mouse Monoclonal 1B9 unconjugated
anti-Polymerase (DNA Directed) iota (POLI) (AA 50-350) antibodyPOLI Reactivity: Human WB Host: Rabbit Polyclonal unconjugated
anti-Polymerase (DNA Directed) iota (POLI) (C-Term) antibodyPOLI Reactivity: Human WB, ELISA Host: Rabbit Polyclonal unconjugated
anti-Polymerase (DNA Directed) iota (POLI) (AA 51-150) antibodyPOLI Reactivity: Rat ELISA, IF (cc), IF (p), IHC (p), ICC, IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Polymerase (DNA Directed) iota (POLI) (AA 143-192) antibodyPOLI Reactivity: Human, Rat, Mouse, Horse, Cow, Zebrafish (Danio rerio), Dog, Guinea Pig, Monkey, Pig, Bat WB Host: Rabbit Polyclonal unconjugated
anti-Polymerase (DNA Directed) iota (POLI) (N-Term) antibodyPOLI Reactivity: Human, Rat, Mouse, Horse, Cow, Zebrafish (Danio rerio), Dog, Guinea Pig, Rabbit, Saccharomyces cerevisiae WB Host: Rabbit Polyclonal unconjugated
anti-Polymerase (DNA Directed) iota (POLI) (AA 673-701), (C-Term) antibodyPOLI Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal unconjugated
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Buffer
- In 1x PBS, pH 7.4
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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: "Both high-fidelity replicative and low-fidelity Y-family polymerases are involved in DNA rereplication." in: Molecular and cellular biology, Vol. 35, Issue 4, pp. 699-715, (2015) (PubMed).
: "Rev1, Rev3, or Rev7 siRNA Abolishes Ultraviolet Light-Induced Translesion Replication in HeLa Cells: A Comprehensive Study Using Alkaline Sucrose Density Gradient Sedimentation." in: Journal of nucleic acids, Vol. 2010, pp. 750296, (2010) (PubMed).
: "Evidence that in xeroderma pigmentosum variant cells, which lack DNA polymerase eta, DNA polymerase iota causes the very high frequency and unique spectrum of UV-induced mutations." in: Cancer research, Vol. 67, Issue 7, pp. 3018-26, (2007) (PubMed).
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: "Both high-fidelity replicative and low-fidelity Y-family polymerases are involved in DNA rereplication." in: Molecular and cellular biology, Vol. 35, Issue 4, pp. 699-715, (2015) (PubMed).
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- Target
- POLI (Polymerase (DNA Directed) iota (POLI))
- Alternative Name
- POLI (POLI Products)
- Background
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Full Gene Name: polymerase (DNA directed) iota
Synonyms: RAD30B,RAD3OB - Gene ID
- 11201
- Pathways
- DNA Damage Repair
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