POLI antibody (AA 616-715)

Catalog No. ABIN564869

Product Details anti-POLI Antibody

Target: POLI (Polymerase (DNA Directed) iota (POLI))

Binding Specificity: AA 616-715

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This POLI antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Mouse monoclonal antibody raised against a partial recombinant POLI.

Sequence: PQGFHFTNSN PAVSAFHSFP NLQSEQLFSR NHTTDSHKQT VATDSHEGLT ENREPDSVDE KITFPSDIDP QVFYELPEAV QKELLAEWKR AGSDFHIGHK

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: POLI (AAH32662, 616 a.a. ~ 715 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone: 8G9

Isotype: IgG3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-POLI antibody (ABIN564869)

Sekimoto, Oda, Kurashima, Hanaoka, Yamashita: "Both high-fidelity replicative and low-fidelity Y-family polymerases are involved in DNA rereplication." in: Molecular and cellular biology, Vol. 35, Issue 4, pp. 699-715, (2015) (PubMed).

Takezawa, Ishimi, Aiba, Yamada: "Rev1, Rev3, or Rev7 siRNA Abolishes Ultraviolet Light-Induced Translesion Replication in HeLa Cells: A Comprehensive Study Using Alkaline Sucrose Density Gradient Sedimentation." in: Journal of nucleic acids, Vol. 2010, pp. 750296, (2010) (PubMed).

Wang, Woodgate, McManus, Mead, McCormick, Maher: "Evidence that in xeroderma pigmentosum variant cells, which lack DNA polymerase eta, DNA polymerase iota causes the very high frequency and unique spectrum of UV-induced mutations." in: Cancer research, Vol. 67, Issue 7, pp. 3018-26, (2007) (PubMed).

Target Details for POLI

Target: POLI (Polymerase (DNA Directed) iota (POLI))

Alternative Name: POLI (POLI Products)

Synonyms: poli antibody, wu:fc27h11 antibody, zgc:110185 antibody, GB12093 antibody, NV16187 antibody, RAD30B antibody, RAD3OB antibody, Rad30b antibody, DNA polymerase iota antibody, polymerase (DNA directed) iota antibody, polymerase (DNA directed), iota antibody, POLI antibody, poli antibody, LOC551219 antibody, AFUA_4G11140 antibody, LOC5568276 antibody, Pol-iota antibody, Poli antibody

Background: Full Gene Name: polymerase (DNA directed) iota
Synonyms: RAD30B,RAD3OB

Gene ID: 11201

Pathways: DNA Damage Repair