APRT antibody (AA 5-49)

Catalog No. ABIN5647397

Product Details anti-APRT Antibody

Target: APRT (Adenine Phosphoribosyltransferase (APRT))

Binding Specificity: AA 5-49

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APRT antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Purification: Antigen affinity purified

Immunogen: Amino acids 5-49 (ELQLVEQRIRSFPDFPTPGVVFRDISPVLKDPASFRAAIGLLARH-human) were used as the immunogen for the APRT antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the APRT antibody should be determined by the researcher.\. Western blot: 0.5-1 μg/mL,FACS: 1-3 μg/10^6 cells

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the APRT antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for APRT

Target: APRT (Adenine Phosphoribosyltransferase (APRT))

Alternative Name: APRT (APRT Products)

Background: Adenine phosphoribosyltransferase (APRTase) is an enzyme encoded by the APRT gene, found in humans on chromosome 16. It belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene.

UniProt: P07741

Pathways: Ribonucleoside Biosynthetic Process