Recombinant gamma H2AX antibody (pSer139)

Catalog No. ABIN5647042

Product Details anti-gamma H2AX Antibody

Target: gamma H2AX (GAMMA-H2AX) (gamma Histone Variant H2AX (GAMMA-H2AX))

Antibody Type: Recombinant Antibody

Binding Specificity: pSer139

Reactivity: All Species

Host: Rabbit

Clonality: Monoclonal

Conjugate: This gamma H2AX antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), ELISA

Characteristics: This recombinant Gamma H2AX antibody reacts to Histone H2A.X only when phosphorylated at serine 139. No cross reactivity with other phosphorylated histones.

Purification: Purified

Immunogen: A phospho-peptide corresponding to phospho-Histone H2AX (S139) was used as the immunogen for this recombinant Gamma H2AX antibody.

Clone: RM224

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the recombinant Gamma H2AX antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western Blot: 0.5-2 μg/mL,ELISA: 0.2-1 μg/mL,Immunocytochemistry: 0.5-2 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the recombinant Gamma H2AX antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).

Target Details for gamma H2AX

Target: gamma H2AX (GAMMA-H2AX) (gamma Histone Variant H2AX (GAMMA-H2AX))

Alternative Name: Gamma H2AX (GAMMA-H2AX Products)

Background: This recombinant Gamma H2AX antibody reacts to Histone H2A.X only when phosphorylated at serine 139. No cross reactivity with other phosphorylated histones.

Gene ID: 3014

UniProt: P16104