USP15 antibody (AA 1-235)

Catalog No. ABIN564282

Product Details anti-USP15 Antibody

Target: USP15 (Ubiquitin Specific Peptidase 15 (USP15))

Binding Specificity: AA 1-235

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This USP15 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Purpose: Mouse monoclonal antibody raised against a full length recombinant USP15.

Sequence: MAEGGAADLD TQRSDIATLL KTSLRKGDTW YLVDSRWFKQ WKKYVGFDSW DKYQMGDQNV YPGPIDNSGL LKDGDAQSLK EHLIDELDYI LLPTEGWNKL VSWYTLMEGQ EPIARKVVEQ GMFVKHCKVE VYLTELKLCE NGNMNNVVTR RFSKADTIDT IEKEIRKIFS IPDEKETRLW NKYMSNTFEP LNKPDSTIQD AGLYQGQVLV IEQKNEDGTW PRGPSTPKKP LEQSC

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: USP15 (AAH20688, 1 a.a. ~ 235 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone: 1C10

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-USP15 antibody (ABIN564282)

Pauli, Chan, Davis, Gableske, Wang, Feister, Gack: "The ubiquitin-specific protease USP15 promotes RIG-I-mediated antiviral signaling by deubiquitylating TRIM25." in: Science signaling, Vol. 7, Issue 307, pp. ra3, (2014) (PubMed).

García-Caballero, Gadotti, Stemkowski, Weiss, Souza, Hodgkinson, Bladen, Chen, Hamid, Pizzoccaro, Deage, François, Bourinet, Zamponi: "The deubiquitinating enzyme USP5 modulates neuropathic and inflammatory pain by enhancing Cav3.2 channel activity." in: Neuron, Vol. 83, Issue 5, pp. 1144-58, (2014) (PubMed).

Cornelissen, Haddad, Wauters, Van Humbeeck, Mandemakers, Koentjoro, Sue, Gevaert, De Strooper, Verstreken, Vandenberghe: "The deubiquitinase USP15 antagonizes Parkin-mediated mitochondrial ubiquitination and mitophagy." in: Human molecular genetics, Vol. 23, Issue 19, pp. 5227-42, (2014) (PubMed).

Fernandez-Sanchez, Sechet, Margottin-Goguet, Rogge, Bianchi: "The human COP9 signalosome protects ubiquitin-conjugating enzyme 3 (UBC3/Cdc34) from beta-transducin repeat-containing protein (betaTrCP)-mediated degradation." in: The Journal of biological chemistry, Vol. 285, Issue 23, pp. 17390-7, (2010) (PubMed).

Vos, Altreuter, White, Howley: "The ubiquitin-specific peptidase USP15 regulates human papillomavirus type 16 E6 protein stability." in: Journal of virology, Vol. 83, Issue 17, pp. 8885-92, (2009) (PubMed).

Xu, Takanashi, Oikawa, Tanaka, Nishi, Isaka, Kudo, Kuroda: "USP15 plays an essential role for caspase-3 activation during Paclitaxel-induced apoptosis." in: Biochemical and biophysical research communications, Vol. 388, Issue 2, pp. 366-71, (2009) (PubMed).

Cayli, Klug, Chapiro, Fröhlich, Krasteva, Orel, Meinhardt: "COP9 signalosome interacts ATP-dependently with p97/valosin-containing protein (VCP) and controls the ubiquitination status of proteins bound to p97/VCP." in: The Journal of biological chemistry, Vol. 284, Issue 50, pp. 34944-53, (2009) (PubMed).

Miyauchi, Kato, Tokunaga, Iwai: "The COP9/signalosome increases the efficiency of von Hippel-Lindau protein ubiquitin ligase-mediated hypoxia-inducible factor-alpha ubiquitination." in: The Journal of biological chemistry, Vol. 283, Issue 24, pp. 16622-31, (2008) (PubMed).

Schweitzer, Bozko, Dubiel, Naumann: "CSN controls NF-kappaB by deubiquitinylation of IkappaBalpha." in: The EMBO journal, Vol. 26, Issue 6, pp. 1532-41, (2007) (PubMed).

Target Details for USP15

Target: USP15 (Ubiquitin Specific Peptidase 15 (USP15))

Alternative Name: USP15 (USP15 Products)

Background: Full Gene Name: ubiquitin specific peptidase 15
Synonyms: KIAA0529,MGC131982,MGC149838,MGC74854,UNPH4

Gene ID: 9958