MFN2 antibody (AA 661-757)

Catalog No. ABIN564263

Product Details anti-MFN2 Antibody

Target: MFN2 (Mitofusin 2 (MFN2))

Binding Specificity: AA 661-757

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MFN2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), RNA Interference (RNAi)

Purpose: Mouse monoclonal antibody raised against a partial recombinant MFN2.

Sequence: FKRQFVEHAS EKLQLVISYT GSNCSHQVQQ ELSGTFAHLC QQVDVTRENL EQEIAAMNKK IEVLDSLQSK AKLLRNKAGW LDSELNMFTH QYLQPSR

Cross-Reactivity: Human, Rat

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: MFN2 (NP_055689, 661 a.a. ~ 757 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone: 4H8

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-MFN2 antibody (ABIN564263)

Chen, Chen, Wang, Tan, Zhu, Li, Han, Chen, Gao, Liu, Chen: "Mitophagy receptor FUNDC1 regulates mitochondrial dynamics and mitophagy." in: Autophagy, Vol. 12, Issue 4, pp. 689-702, (2016) (PubMed).

Esteves, G-Fernandes, Santos, Januário, Cardoso: "The Upshot of LRRK2 Inhibition to Parkinson's Disease Paradigm." in: Molecular neurobiology, (2015) (PubMed).

Fiuza-Luces, Delmiro, Soares-Miranda, González-Murillo, Martínez-Palacios, Ramírez, Lucia, Morán: "Exercise training can induce cardiac autophagy at end-stage chronic conditions: insights from a graft-versus-host-disease mouse model." in: Brain, behavior, and immunity, Vol. 39, pp. 56-60, (2014) (PubMed).

Lally, Herbst, Matravadia, Maher, Perry, Ventura-Clapier, Holloway: "Over-expressing mitofusin-2 in healthy mature mammalian skeletal muscle does not alter mitochondrial bioenergetics." in: PLoS ONE, Vol. 8, Issue 1, pp. e55660, (2013) (PubMed).

Russell, Lamon, Boon, Wada, Güller, Brown, Chibalin, Zierath, Snow, Stepto, Wadley, Akimoto: "Regulation of miRNAs in human skeletal muscle following acute endurance exercise and short-term endurance training." in: The Journal of physiology, Vol. 591, Issue Pt 18, pp. 4637-53, (2013) (PubMed).

Cosson, Marchetti, Ravazzola, Orci: "Mitofusin-2 independent juxtaposition of endoplasmic reticulum and mitochondria: an ultrastructural study." in: PLoS ONE, Vol. 7, Issue 9, pp. e46293, (2012) (PubMed).

Morán, Rivera, Sánchez-Aragó, Blázquez, Merinero, Ugalde, Arenas, Cuezva, Martín: "Mitochondrial bioenergetics and dynamics interplay in complex I-deficient fibroblasts." in: Biochimica et biophysica acta, Vol. 1802, Issue 5, pp. 443-53, (2010) (PubMed).

Holloway, Perry, Thrush, Heigenhauser, Dyck, Bonen, Spriet: "PGC-1alpha's relationship with skeletal muscle palmitate oxidation is not present with obesity despite maintained PGC-1alpha and PGC-1beta protein." in: American journal of physiology. Endocrinology and metabolism, Vol. 294, Issue 6, pp. E1060-9, (2008) (PubMed).

Target Details for MFN2

Target: MFN2 (Mitofusin 2 (MFN2))

Alternative Name: MFN2 (MFN2 Products)

Background: Full Gene Name: mitofusin 2
Synonyms: CMT2A,CMT2A2,CPRP1,HSG,KIAA0214,MARF

Gene ID: 9927

NCBI Accession: NM_014874

Pathways: Skeletal Muscle Fiber Development