USP9X antibody (AA 1-90)

Catalog No. ABIN563570

Product Details anti-USP9X Antibody

Target: USP9X (Ubiquitin Specific Peptidase 9, X-Linked (USP9X))

Binding Specificity: AA 1-90

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This USP9X antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Purpose: Mouse monoclonal antibody raised against a partial recombinant USP9X.

Sequence: MTATTRGSPV GGNDNQGQAP DGQSQPPLQQ NQTSSPDSSN ENSPATPPDE QGQGDAPPQL EDEEPAFPHT DLAKLDDMIN RPRWVVPVLP

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: USP9X (NP_068706, 1 a.a. ~ 90 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone: 1C4

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-USP9X antibody (ABIN563570)

Kim, Kim, Park, Lee, Lim: "Role of Angiomotin-like 2 mono-ubiquitination on YAP inhibition." in: EMBO reports, Vol. 17, Issue 1, pp. 64-78, (2016) (PubMed).

Wolfsperger, Hogh-Binder, Schittenhelm, Psaras, Ritter, Bornes, Huber, Jendrossek, Rudner: "Deubiquitylating enzyme USP9x regulates radiosensitivity in glioblastoma cells by Mcl-1-dependent and -independent mechanisms." in: Cell death & disease, Vol. 7, pp. e2039, (2016) (PubMed).

Wang, Xie, Li, Owonikoko, Ramalingam, Khuri, Curran, Wang, Deng: "Role of Ku70 in deubiquitination of Mcl-1 and suppression of apoptosis." in: Cell death and differentiation, Vol. 21, Issue 7, pp. 1160-9, (2014) (PubMed).

Mazumder, Choudhary, Al-Harbi, Almasan: "Mcl-1 Phosphorylation defines ABT-737 resistance that can be overcome by increased NOXA expression in leukemic B cells." in: Cancer research, Vol. 72, Issue 12, pp. 3069-79, (2012) (PubMed).

Trivigno, Essmann, Huber, Rudner: "Deubiquitinase USP9x confers radioresistance through stabilization of Mcl-1." in: Neoplasia (New York, N.Y.), Vol. 14, Issue 10, pp. 893-904, (2012) (PubMed).

Hikita, Takehara, Shimizu, Kodama, Shigekawa, Iwase, Hosui, Miyagi, Tatsumi, Ishida, Li, Kanto, Hiramatsu, Hayashi: "The Bcl-xL inhibitor, ABT-737, efficiently induces apoptosis and suppresses growth of hepatoma cells in combination with sorafenib." in: Hepatology (Baltimore, Md.), Vol. 52, Issue 4, pp. 1310-21, (2010) (PubMed).

Target Details for USP9X

Target: USP9X (Ubiquitin Specific Peptidase 9, X-Linked (USP9X))

Alternative Name: USP9X (USP9X Products)

Background: Full Gene Name: ubiquitin specific peptidase 9, X-linked
Synonyms: DFFRX,FAF,FAM

Gene ID: 8239

NCBI Accession: NM_021906