STIM1 antibody (AA 24-685)

Catalog No. ABIN563045

Product Details anti-STIM1 Antibody

Target: STIM1 (Stromal Interaction Molecule 1 (STIM1))

Binding Specificity: AA 24-685

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This STIM1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Purpose: Mouse monoclonal antibody raised against a full length recombinant STIM1.

Sequence: SHSHSEKATG TSSGANSEES TAAEFCRIDK PLCHSEDEKL SFEAVRNIHK LMDDDANGDV DVEESDEFLR EDLNYHDPTV KHSTFHGEDK LISVEDLWKA WKSSEVYNWT VDEVVQWLIT YVELPQYEET FRKLQLSGHA MPRLAVTNTT MTGAVLKMTD RSHRQKLQLK ALDTVLFGPP LLTRHNHLKD FMLVVSIVIG VGGCWFAYIQ NRYSKEHMKK MMKDLEGLHR AEQSLHDLQE RLHKAQEEHR TVEVEKVHLE KKLRDEINLA KQEAQRLKEL REGTENERSR QKYAEEELEQ VREALRKAEK ELESHSSWYA PEALQKWLQL THEVEVQYYN IKKQNAEKQL LVAKEGAEKI KKKRNTLFGT FHVAHSSSLD DVDHKILTAK QALSEVTAAL RERLHRWQQI EILCGFQIVN NPGIHSLVAA LNIDPSWMGS TRPNPAHFIM TDDVDDMDEE IVSPLSMQSP SLQSSVRQRL TEPQHGLGSQ RDLTHSDSES SLHMSDRQRV APKPPQMSRA ADEALNAMTS NGSHRLIEGV HPGSLVEKLP DSPALAKKAL LALNHGLDKA HSLMELSPSA PPGGSPHLDS SRSHSPSSPD PDTPSPVGDS RALQASRNTR IPHLAGKKAV AEEDNGSIGE ETDSSPGRKK FPLKIFKKPL KK

Cross-Reactivity: Human, Rat

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: STIM1 (AAH21300, 24 a.a. ~ 685 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone: 5A2

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-STIM1 antibody (ABIN563045)

Hong, Chang, Wang, Yu, Lee: "IL-9 induces IL-8 production via STIM1 activation and ERK phosphorylation in epidermal keratinocytes: A plausible mechanism of IL-9R in atopic dermatitis." in: Journal of dermatological science, Vol. 78, Issue 3, pp. 206-14, (2015) (PubMed).

Umemura, Baljinnyam, Feske, De Lorenzo, Xie, Feng, Oda, Makino, Fujita, Yokoyama, Iwatsubo, Chen, Goydos, Ishikawa, Iwatsubo: "Store-operated Ca2+ entry (SOCE) regulates melanoma proliferation and cell migration." in: PLoS ONE, Vol. 9, Issue 2, pp. e89292, (2014) (PubMed).

Ferdaus, Xiao, Ohara, Nemoto, Harada, Saar, Hübner, Isomura, Nabika: "Identification of Stim1 as a candidate gene for exaggerated sympathetic response to stress in the stroke-prone spontaneously hypertensive rat." in: PLoS ONE, Vol. 9, Issue 4, pp. e95091, (2014) (PubMed).

Jans, Mottram, Johnson, Brown, Sikkink, Ross, Reynolds: "Lysophosphatidic acid promotes cell migration through STIM1- and Orai1-mediated Ca2+(i) mobilization and NFAT2 activation." in: The Journal of investigative dermatology, Vol. 133, Issue 3, pp. 793-802, (2013) (PubMed).

Eylenstein, Schmidt, Gu, Yang, Schmid, Schmidt, Alesutan, Szteyn, Regel, Shumilina, Lang: "Transcription factor NF-?B regulates expression of pore-forming Ca2+ channel unit, Orai1, and its activator, STIM1, to control Ca2+ entry and affect cellular functions." in: The Journal of biological chemistry, Vol. 287, Issue 4, pp. 2719-30, (2012) (PubMed).

Elvers, Herrmann, Seizer, Münzer, Beck, Schönberger, Borst, Martin-Romero, Lang, May, Gawaz: "Intracellular cyclophilin A is an important Ca(2+) regulator in platelets and critically involved in arterial thrombus formation." in: Blood, Vol. 120, Issue 6, pp. 1317-26, (2012) (PubMed).

Henke, Albrecht, Pfeiffer, Toutzaris, Zanger, Methner: "Stromal interaction molecule 1 (STIM1) is involved in the regulation of mitochondrial shape and bioenergetics and plays a role in oxidative stress." in: The Journal of biological chemistry, Vol. 287, Issue 50, pp. 42042-52, (2012) (PubMed).

Schmid, Bhandaru, Nurbaeva, Yang, Szteyn, Russo, Leibrock, Tyan, Pearce, Shumilina, Lang: "SGK3 regulates Ca(2+) entry and migration of dendritic cells." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 30, Issue 6, pp. 1423-35, (2012) (PubMed).

Kiviluoto, Decuypere, De Smedt, Missiaen, Parys, Bultynck: "STIM1 as a key regulator for Ca2+ homeostasis in skeletal-muscle development and function." in: Skeletal muscle, Vol. 1, Issue 1, pp. 16, (2011) (PubMed).

Steinbeck, Henke, Opatz, Gruszczynska-Biegala, Schneider, Theiss, Hamacher, Steinfarz, Golz, Brüstle, Kuznicki, Methner: "Store-operated calcium entry modulates neuronal network activity in a model of chronic epilepsy." in: Experimental neurology, Vol. 232, Issue 2, pp. 185-94, (2011) (PubMed).

Schuhmann, Stegner, Berna-Erro, Bittner, Braun, Kleinschnitz, Stoll, Wiendl, Meuth, Nieswandt: "Stromal interaction molecules 1 and 2 are key regulators of autoreactive T cell activation in murine autoimmune central nervous system inflammation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 3, pp. 1536-42, (2010) (PubMed).

Saitoh, Oritani, Saito, Yokota, Ichii, Sudo, Fujita, Nakajima, Okada, Kanakura: "Identification of functional domains and novel binding partners of STIM proteins." in: Journal of cellular biochemistry, Vol. 112, Issue 1, pp. 147-56, (2010) (PubMed).

Braun, Gessner, Varga-Szabo, Syed, Konrad, Stegner, Vögtle, Schmidt, Nieswandt: "STIM1 is essential for Fcgamma receptor activation and autoimmune inflammation." in: Blood, Vol. 113, Issue 5, pp. 1097-104, (2009) (PubMed).

Wu, Wang, Young, Lai, Li: "Proteome-wide identification of novel binding partners to the oncogenic fusion gene protein, NPM-ALK, using tandem affinity purification and mass spectrometry." in: The American journal of pathology, Vol. 174, Issue 2, pp. 361-70, (2009) (PubMed).

Beyersdorf, Braun, Vögtle, Varga-Szabo, Galdos, Kissler, Kerkau, Nieswandt: "STIM1-independent T cell development and effector function in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 6, pp. 3390-7, (2009) (PubMed).

Pani, Ong, Brazer, Liu, Rauser, Singh, Ambudkar: "Activation of TRPC1 by STIM1 in ER-PM microdomains involves release of the channel from its scaffold caveolin-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 47, pp. 20087-92, (2009) (PubMed).

Alicia, Angélica, Carlos, Alfonso, Vaca: "STIM1 converts TRPC1 from a receptor-operated to a store-operated channel: moving TRPC1 in and out of lipid rafts." in: Cell calcium, Vol. 44, Issue 5, pp. 479-91, (2008) (PubMed).

Pani, Ong, Liu, Rauser, Ambudkar, Singh: "Lipid rafts determine clustering of STIM1 in endoplasmic reticulum-plasma membrane junctions and regulation of store-operated Ca2+ entry (SOCE)." in: The Journal of biological chemistry, Vol. 283, Issue 25, pp. 17333-40, (2008) (PubMed).

Varga-Szabo, Braun, Kleinschnitz, Bender, Pleines, Pham, Renné, Stoll, Nieswandt: "The calcium sensor STIM1 is an essential mediator of arterial thrombosis and ischemic brain infarction." in: The Journal of experimental medicine, Vol. 205, Issue 7, pp. 1583-91, (2008) (PubMed).

Grosse, Braun, Varga-Szabo, Beyersdorf, Schneider, Zeitlmann, Hanke, Schropp, Mühlstedt, Zorn, Huber, Schmittwolf, Jagla, Yu, Kerkau, Schulze, Nehls, Nieswandt: "An EF hand mutation in Stim1 causes premature platelet activation and bleeding in mice." in: The Journal of clinical investigation, Vol. 117, Issue 11, pp. 3540-50, (2007) (PubMed).

Target Details for STIM1

Target: STIM1 (Stromal Interaction Molecule 1 (STIM1))

Alternative Name: STIM1 (STIM1 Products)

Synonyms: stim1 antibody, MGC76045 antibody, STIM1 antibody, si:dkey-24p1.5 antibody, MGC154748 antibody, si:ch211-112l17.2 antibody, Stim1 antibody, D11S4896E antibody, GOK antibody, TAM antibody, SIM antibody, stromal interaction molecule 1 antibody, stromal interaction molecule 1a antibody, stromal interaction molecule 1 L homeolog antibody, stromal interaction molecule 1b antibody, STIM1 antibody, stim1 antibody, stim1a antibody, stim1.L antibody, stim1b antibody, Stim1 antibody

Background: Full Gene Name: stromal interaction molecule 1
Synonyms: D11S4896E,GOK

Gene ID: 6786

Pathways: TCR Signaling, BCR Signaling