RPS19 antibody (AA 1-145)

Catalog No. ABIN562734

Product Details anti-RPS19 Antibody

Target: RPS19 (Ribosomal Protein S19 (RPS19))

Binding Specificity: AA 1-145

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RPS19 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Mouse monoclonal antibody raised against a full length recombinant RPS19.

Sequence: MPGVTVKDVN QQEFVRALAA FLKKSGKLKV PEWVDTVKLA KHKELAPYDE NWFYTRAAST ARHLYLRGGA GVGSMTKIYG GRQRNGVMPS HFSRGSKSVA RRVLQALEGL KMVEKDQDGG RKLTPQGQRD LDRIAGQVAA ANKKH

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: RPS19 (AAH00023, 1 a.a. ~ 145 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone: 3C6

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-RPS19 antibody (ABIN562734)

Singh, Goldberg, Henson, Husain-Krautter, Nihrane, Blanc, Ellis, Lipton, Liu: "p53-Independent cell cycle and erythroid differentiation defects in murine embryonic stem cells haploinsufficient for Diamond Blackfan anemia-proteins: RPS19 versus RPL5." in: PLoS ONE, Vol. 9, Issue 2, pp. e89098, (2014) (PubMed).

Aspesi, Pavesi, Robotti, Crescitelli, Boria, Avondo, Moniz, Da Costa, Mohandas, Roncaglia, Ramenghi, Ronchi, Gustincich, Merlin, Marengo, Ellis, Follenzi, Santoro, Dianzani: "Dissecting the transcriptional phenotype of ribosomal protein deficiency: implications for Diamond-Blackfan Anemia." in: Gene, Vol. 545, Issue 2, pp. 282-9, (2014) (PubMed).

Caterino, Corbo, Imperlini, Armiraglio, Pavesi, Aspesi, Loreni, Dianzani, Ruoppolo: "Differential proteomic analysis in human cells subjected to ribosomal stress." in: Proteomics, Vol. 13, Issue 7, pp. 1220-7, (2013) (PubMed).

Sezgin, Henson, Nihrane, Singh, Wattenberg, Alard, Ellis, Liu: "Impaired growth, hematopoietic colony formation, and ribosome maturation in human cells depleted of Shwachman-Diamond syndrome protein SBDS." in: Pediatric blood & cancer, Vol. 60, Issue 2, pp. 281-6, (2012) (PubMed).

Rey, Duffy, Brown, Kennedy, Dick, Dror, Tailor: "Enhanced alternative splicing of the FLVCR1 gene in Diamond Blackfan anemia disrupts FLVCR1 expression and function that are critical for erythropoiesis." in: Haematologica, Vol. 93, Issue 11, pp. 1617-26, (2008) (PubMed).

Target Details for RPS19

Target: RPS19 (Ribosomal Protein S19 (RPS19))

Alternative Name: RPS19 (RPS19 Products)

Background: Full Gene Name: ribosomal protein S19
Synonyms: DBA

Gene ID: 6223

Pathways: Positive Regulation of Immune Effector Process, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly