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SMAD3 antibody (AA 120-221)

Name: SMAD3 antibody (AA 120-221)
SKU: ABIN561715
Price: 651.43 USD
Availability: InStock

SMAD3 Reactivity: Human WB, ELISA, IHC (p), PLA Host: Mouse Monoclonal 7F3 unconjugated

SMAD3 monoclonal antibody (M02), clone 7F3. (SMAD3 antibody (AA 120-221))

Western Blot analysis of SMAD3 expression in transfected 293T cell line by SMAD3 monoclonal antibody (M02), clone 7F3. (SMAD3 antibody (AA 120-221))

Immunoperoxidase of monoclonal antibody to SMAD3 on formalin-fixed paraffin-embedded human placenta. (SMAD3 antibody (AA 120-221))

ISt

Western Blot detection against Immunogen (36. (SMAD3 antibody (AA 120-221))

Detection limit for recombinant GST tagged SMAD3 is approximately 0. (SMAD3 antibody (AA 120-221))

ELISA

Proximity Ligation Analysis of protein-protein interactions between MAPK8 and SMAD3. (SMAD3 antibody (AA 120-221))

PLA

10 images

(1 reference)

Catalog No. ABIN561715

Datasheet as PDF

Target See all SMAD3 Antibodies

SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))
Binding Specificity
38

32

32

30

25

14

12

9

8

7

6

6

5

5

5

5

4

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 120-221
Reactivity
252

168

151

29

12

10

9

8

8

6

3

3

2

2

1

1

1
Human
Host
243

22
Mouse
Compatible Secondaries
Clonality
231

34
Monoclonal
Conjugate
122

15

14

13

10

7

6

6

6

6

6

6

4

4

4

4

4

4

4

4

4

4

4

1

1

1

1
This SMAD3 antibody is un-conjugated
Application
202

105

88

74

55

54

53

50

27

13

11

8

5

4

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Proximity Ligation Assay (PLA)

Purpose

Mouse monoclonal antibody raised against a partial recombinant SMAD3.

Sequence

VCVNPYHYQR VETPVLPPVL VPRHTEIPAE FPPLDDYSHS IPENTNFPAG IEPQSNIPET PPPGYLSEDG ETSDHQMNHS MDAGSPNLSP NPMSPAHNNL DL

Cross-Reactivity

Human, Rat

Characteristics

Antibody Reactive Against Recombinant Protein.

Immunogen

SMAD3 (NP_005893, 120 a.a. ~ 221 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone

7F3

Isotype

IgG1

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SMAD3 Reactivity: Human, Mouse, Rat WB, IHC (p), FACS, IF (cc) Host: Rabbit Monoclonal 3D1 unconjugated

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KO Validated SMAD3 Reactivity: Human WB, IHC, IF, IP Host: Rabbit Monoclonal unconjugated

Application Notes

Optimal working dilution should be determined by the investigator.

Restrictions

For Research Use only
Buffer

In 1x PBS, pH 7.4

Handling Advice

Aliquot to avoid repeated freezing and thawing.

Storage

-20 °C

Storage Comment

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Nagel, Ehrentraut, Tomasch, Quentmeier, Meyer, Kaufmann, Drexler, MacLeod: "Ectopic expression of homeobox gene NKX2-1 in diffuse large B-cell lymphoma is mediated by aberrant chromatin modifications." in: PLoS ONE, Vol. 8, Issue 4, pp. e61447, (2013) (PubMed).
Target

SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Alternative Name

SMAD3 (SMAD3 Products)

Synonyms

HSPC193 antibody, HsT17436 antibody, JV15-2 antibody, LDS1C antibody, LDS3 antibody, MADH3 antibody, madh3 antibody, madh3a antibody, smad3 antibody, wu:fa99e03 antibody, XSmad3 antibody, XenMLP antibody, Xmad3 antibody, madh3-A antibody, Madh3 antibody, AU022421 antibody, Smad 3 antibody, mad3 antibody, madh3b antibody, zgc:92234 antibody, SMAD family member 3 antibody, SMAD family member 3a antibody, SMAD family member 3 L homeolog antibody, SMAD family member 3b antibody, SMAD3 antibody, smad3a antibody, smad3.L antibody, Smad3 antibody, smad3b antibody

Background

Full Gene Name: SMAD family member 3
Synonyms: DKFZp586N0721,DKFZp686J10186,HSPC193,HsT17436,JV15-2,MADH3,MGC60396

Gene ID

4088

NCBI Accession

NM_005902

Pathways

Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy