H3F3B antibody (AA 1-136)

Catalog No. ABIN561220

Product Details anti-H3F3B Antibody

Target: H3F3B (H3 Histone, Family 3B (H3.3B) (H3F3B))

Binding Specificity: AA 1-136

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This H3F3B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Mouse monoclonal antibody raised against a full length recombinant H3F3B.

Sequence: MARTKQTARK STGGKAPRKQ LATKAARKSA PSTGGVKKPH RYRPGTVALR EIRRYQKSTE LLIRKLPFQR LVREIAQDFK TDLRFQSAAI GALQEASEAY LVGLFEDTNL CAIHAKRVTI MPKDIQLARR IRGERA

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: H3F3B (AAH17558, 1 a.a. ~ 136 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone: 2D7-H1

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-H3F3B antibody (ABIN561220)

Hammoud, Low, Yi, Carrell, Guccione, Cairns: "Chromatin and transcription transitions of mammalian adult germline stem cells and spermatogenesis." in: Cell stem cell, Vol. 15, Issue 2, pp. 239-53, (2014) (PubMed).

Jullien, Astrand, Szenker, Garrett, Almouzni, Gurdon: "HIRA dependent H3.3 deposition is required for transcriptional reprogramming following nuclear transfer to Xenopus oocytes." in: Epigenetics & chromatin, Vol. 5, Issue 1, pp. 17, (2013) (PubMed).

Orsi, Algazeery, Meyer, Capri, Sapey-Triomphe, Horard, Gruffat, Couble, Aït-Ahmed, Loppin: "Drosophila Yemanuclein and HIRA cooperate for de novo assembly of H3.3-containing nucleosomes in the male pronucleus." in: PLoS genetics, Vol. 9, Issue 2, pp. e1003285, (2013) (PubMed).

Lin, Conti, Ramalho-Santos: "Histone variant H3.3 maintains a decondensed chromatin state essential for mouse preimplantation development." in: Development (Cambridge, England), Vol. 140, Issue 17, pp. 3624-34, (2013) (PubMed).

Szenker, Lacoste, Almouzni: "A developmental requirement for HIRA-dependent H3.3 deposition revealed at gastrulation in Xenopus." in: Cell reports, Vol. 1, Issue 6, pp. 730-40, (2012) (PubMed).

Drané, Ouararhni, Depaux, Shuaib, Hamiche: "The death-associated protein DAXX is a novel histone chaperone involved in the replication-independent deposition of H3.3." in: Genes & development, Vol. 24, Issue 12, pp. 1253-65, (2010) (PubMed).

Target Details for H3F3B

Target: H3F3B (H3 Histone, Family 3B (H3.3B) (H3F3B))

Alternative Name: H3F3B (H3F3B Products)

Background: Full Gene Name: H3 histone, family 3B (H3.3B)
Synonyms: H3.3B,H3F3A

Gene ID: 3021