ELANE antibody (AA 140-267)

Catalog No. ABIN5611333

Product Details anti-ELANE Antibody

Target: ELANE (Elastase, Neutrophil Expressed (ELANE))

Binding Specificity: AA 140-267

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ELANE antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Purpose: ELANE Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human ELANE (AA: 140-267) expressed in E. Coli.

Clone: 6B6B10

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Target Details for ELANE

Target: ELANE (Elastase, Neutrophil Expressed (ELANE))

Alternative Name: ELANE (ELANE Products)

Synonyms: ELA2 antibody, GE antibody, HLE antibody, HNE antibody, NE antibody, PMN-E antibody, SCN1 antibody, Ela2 antibody, F430011M15Rik antibody, ela2 antibody, ELANE antibody, elastase, neutrophil expressed antibody, proteinase 3 antibody, ELANE antibody, Elane antibody, prtn3 antibody

Background:

Description: Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19.

Aliases: GE, NE, HLE, HNE, ELA2, SCN1, PMN-E

Molecular Weight: 28.5kDa

Gene ID: 1991

HGNC: 1991

UniProt: P08246