APAF1 antibody (AA 1138-1237)

Catalog No. ABIN5611196

Product Details anti-APAF1 Antibody

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Binding Specificity: AA 1138-1237

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This APAF1 antibody is un-conjugated

Application: ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Purpose: APAF1 Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human APAF1 (AA: 1138-1237) expressed in E. Coli.

Clone: 2H9A1

Isotype: IgG2b

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/100 - 1/500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Target Details for APAF1

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Alternative Name: APAF1 (APAF1 Products)

Background:

Description: This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.

Aliases: CED4, APAF-1

Molecular Weight: 141.8kDa

Gene ID: 317

HGNC: 317

Pathways: p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity