MPL antibody (AA 26-175)

Catalog No. ABIN5611182

Product Details anti-MPL Antibody

Target: MPL (Myeloproliferative Leukemia Virus Oncogene (MPL))

Binding Specificity: AA 26-175

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MPL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Neutralization (Neut)

Purification: purified

Immunogen: Purified recombinant fragment of human CD110 (AA: extra 26-175) expressed in E. coli.

Clone: 1D6B7

Isotype: IgG2a

Application Details

Application Notes: ELISA: 1:10000, WB: 1:500 - 1:2000, ICC: N/A, FCM: 1:200 - 1:400, IHC: N/A

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: 4°C, -20°C for long term storage

Target Details for MPL

Target: MPL (Myeloproliferative Leukemia Virus Oncogene (MPL))

Alternative Name: CD110 (MPL Products)

Synonyms: C-MPL antibody, CD110 antibody, MPLV antibody, THCYT2 antibody, TPOR antibody, TPO-R antibody, c-mpl antibody, c-mpl-I antibody, c-mpl-II antibody, hlb219 antibody, MPL antibody, mpll antibody, tpor antibody, tpor.b antibody, MPL proto-oncogene, thrombopoietin receptor antibody, myeloproliferative leukemia virus oncogene antibody, MPL antibody, Mpl antibody, mpl antibody

Background:

Description: In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs . TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated.

Aliases: MPL, MPLV, TPOR, C-MPL, THCYT2

Molecular Weight: 71.2 kDa

Gene ID: 4352

HGNC: 4352

Pathways: JAK-STAT Signaling